摘要
目的:研究布洛芬与双氢青蒿素配伍对类风湿关节炎的治疗效果,初步探讨其配伍机制。方法:选择体重160～180 g的SD大鼠随机分为7组:正常对照组、模型对照组、甲氨蝶呤对照组、配伍高剂量治疗组、配伍低剂量治疗组、布洛芬治疗组、双氢青蒿素治疗组。采用佐剂性关节炎(adjuvant arthritis,AA)动物模型,取踝关节进行病理学观察,并运用流式细胞术检测外周血T淋巴细胞亚群、酶联免疫法检测血清白介素-4(IL-4),干扰素-γ(IFN-γ)的水平。结果:配伍组能显著下调外周血CD 4+T细胞百分比和CD 4+T/CD 8+T比值(P<0.01),下调血清INF-γ水平(P<0.01)、上调IL-4水平(P<0.01);与布洛芬组比较,双氢青蒿素组上调IL-4有明显优势(P<0.01),配伍组比单用药物能更有效延缓模型鼠的关节病理损伤。结论:布洛芬与双氢青蒿素配伍能抑制CD 4+T增殖及促进Th1/Th2向右漂移并形成互补,进而增强关节炎治疗效果。
Objective：To study the therapeutic effects of the combination of ibuprofen and dihydroartemisinin on rheumatoid arthritis in rats,and to explore the compatible mechanism of the drugs.Method： Standard Sprague Dawley rats with weight of 160-180 g were randomly divided into 7 groups,including normal group,adjuvant arthritis（AA） model group,methotrexate group,Compatibility high-dose group,Compatibility low-dose group,ibuprofen group,dihydroartemisinine group.We detected T-lymphocyte subsets in peripheral blood by flow cytometry,and measured the levels of INF-γ and IL-4 in serum by ELISA for AA animal model treated by drugs.Result：The combination group could significantly reduced the percentage of CD 4＋ T cells and CD 4＋ T / CD 8＋ T ratio in the peripheral blood lymphocytes cells,reduce INF-γ and improve IL-4 in serum.Compared with the ibuprofen group,dihydroartemisinin could significantly improve IL-4（P 0.01）.The combination of the drugs could more effectively delay the joint injury of the model rats compared with those given individual drug.Conclusion： The combination of ibuprofen with dihydroartemisinin can suppress proliferation of CD4 ＋ T cells,and promote the Th1 /Th2 shift towards the Th2 cytokine response,and can complement,thus enhancing the therapeutic effect for arthritis.
出处
《中国实验方剂学杂志》
CAS
北大核心
2011年第9期177-180,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
863计划项目(2003AA2Z3514)
江西省教育厅科技项目(GJJ08409)
关键词
布洛芬
双氢青蒿素
配伍
佐剂性关节炎
ibuprofen
dihydroartemisinin
combination
adjuvant arthritis
