摘要
以刺五加试管苗叶片为试材,将叶片接种于含有不同种类和浓度激素的培养基上进行培养,研究不同激素对刺五加胚性愈伤组织诱导、胚状体发生及生根的影响。结果表明:诱导叶片胚性愈伤组织发生的最适培养基为MS+6-BA 1.5 mg.L-1+2,4-D 1.0 mg.L-1,胚状体生长发育的最适培养基为MS+6-BA1.0 mg.L-1+IBA0.5 mg.L-1,生根的最适培养基为1/2MS+NAA0.4 mg.L-1。
The effect of plant growth regulating substances on embryonic callus induction,somatic embryogenesis and rootting were investigated by means of vitro culture from leaf of test-tube seedlings of Acanthopanax senticosus.The results showed that the optimum medium on embryogenic callus induction was MS＋6-BA 1.5 mg·L-1＋2,4-D 1.0 mg·L-1.The optimum medium on growth and development of somatic embryo was MS＋6-BA 1.0 mg·L-1＋IBA 0.5 mg·L-1.The optimum medium on rootting was1/2MS＋NAA 0.4 mg·L-1.
出处
《辽宁林业科技》
2011年第2期8-9,13,共3页
Liaoning Forestry Science and Technology
关键词
刺五加
胚状体
成苗
Acanthopanax senticosus
somatic embryo
seedling
