摘要
参考GenBank发表的猪圆环病毒2型（Porcine circovirus type 2,PCV2）全基因组序列,设计引物,通过PCR技术,从广东地区疑似＂高热病＂病猪的组织中,扩增17株PCV2流行株的全基因,并进行了序列测定分析.结果表明,17个PCV2分离株基因组全长为1 767或1 768 bp.相似性比较发现,17个分离株之间的核苷酸相似性为96.7%-99.8%,与GenBank上已发表的国外分离株比较,相似性为95.6%-99.8%;17个分离株ORF2、ORF3的核苷酸相似性分别为89.5%-100%和97.5%-100%;系统发育树显示,17个毒株有2株属于a亚群,10株属于b亚群,5株属于d亚群.
According to the sequences of porcine circovirus type 2（PCV2） isolates in GenBank,the genomes were amplified by PCR from the tissues in suspect swine＂high fever＂ from Guangdong province,followed by sequencing and analysis.The results showed that the complete genome of each of seventeen isolates were 1 767 or 1 768 bp in length.There were 96.7% to 99.8% homologies among the complete genomic nucleotides of seventeen isolates,and 96.7% to 99.8% homologies among isolates abroad in GenBank.The ORF2 of the seventeen isolates had 89.5% to 100% nucleotide sequence homologies and 97.5% to 100% amino acid homologies.Phylogenetic analysis showed that two strains belong to a subtypes,ten strains belong to the b subtypes and five strains belong to the d subtypes among the total seventeen.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2011年第2期89-92,共4页
Journal of South China Agricultural University
基金
国家现代农业产业技术体系项目(nyctx009)
863计划项目(2006AA10A204)
关键词
猪圆环病毒2型
基因克隆
序列分析
porcine circovirus type 2
gene cloning
sequence analysis
