摘要
目的:探讨CCNA1基因甲基化在乳腺癌发生发展中的作用。方法:采用Qiagen-FFPE的步骤提取95例石蜡包埋乳腺癌组织的基因组DNA,应用限制性酶切-PCR对所提DNA进行CCNA1甲基化检测。结果:乳腺癌组织中CCNA1基因启动子甲基化的阳性率为90.9%(86/95)。结论:CCNA1基因甲基化参与乳腺癌的发生,发展,有淋巴结转移多见的特征,CCNA1基因甲基化与乳腺癌转移由关。
Objective：To investigate the methylation pattern of CCNA1 in breast carcinoma and explore the role of these epige-netic changes in tumorgenensis.Methods：95 specimens of breast cancer were used,and the DNA was extracted by Qiagen procedure.Methylation change in the promoter region of CCNA1 was determined by using restricted enzyme cut-PCR.Results：The positive rate of gene promoter hypermethylation of CCNA1 was 90.9%（86/95）.Conclusions：CCNA1 hypermethylation was correlated with lymph node metastasis,which may play an important role in pathogenesis of breast cancer.
出处
《现代生物医学进展》
CAS
2011年第7期1321-1323,1326,共4页
Progress in Modern Biomedicine
