摘要
通过进一步开发QE6500光纤光谱仪在测定植物叶绿素荧光方面的功能,研究单色光质和混合光质对黄瓜叶片叶绿素荧光的影响,以期在光质对植物光合影响方面和植物补光方面有新的突破。试验以北方白黄瓜幼苗为试材,用蓝、绿、红发光二级管(LED)单色光进行单色光和混合光照射诱导处理,分析光质对Fp685、Fp735、Fp685/Fp735、F685、F735、F685/F735和Y(II)的影响。结果表明,3种LED单色光源照射诱导下,随着光强的增加白黄瓜叶片叶绿素荧光曲线OJIP中P点呈现快速上升趋势。其中,Fp685主要表现为蓝光>绿光>红光;而Fp735均表现为绿光下高,红蓝光下低;Fp685/Fp735荧光比值均表现为蓝光最高,绿光和红光下较低。混合光得到的荧光曲线的P点出现早于单色光照射,且P混合>P单色光加和。混合光荧光值计算得到的F685/F735比值始终低于单色光荧光加和值计算得到的F685/F735比值。各个单色光质下的PSⅡ实际光化学量子产量表现为:Y(II)绿光>Y(II)红光>Y(II)蓝光。蓝光下黄瓜光系统活性较高,绿光下光合能力最强,进行植物红蓝混合补光时加入绿光会明显提高植物的光和能力;可以使用QE65000光纤光谱仪进行植物荧光测定。
Through further development of QE6500 fiber spectrometer in the determination of chlorophyll fluorescence such functions,research monochromatic light quality and mixing of light quality on cucumber chlorophyll fluorescence,in order to make breakthrough on the light quality on plant photosynthesis impact and plants fill light.The objective of this study was to analyze the effect of light qualities on Fp685、Fp735、Fp685/Fp735、F685、F735、F685/F735 and Y（II）of chlorophyll fluorescence in green leaves green leaves induced by blue,green and red light-emitting diode（LED）in northern white cucumber seeding.With the increase of light intensity,the P in OJIP of chlorophyll fluorescence curve showed a fast rising.Fp685 were in the order of red bluegreen,but Fp735 were in the order of green red≈ blue.However,the order of blue green≈ red were observed for the ratio of Fp685/Fp735.The point of P appeared earlier under the mixed light than under single light,and PmixPsingle.The ratios of F685/F735 always lower under the mixed lights than under single light.The order of Y（II）green Y（II）red Y（II）blue were observed for the yield of PSⅡ by single light.Conclusion：The photosystem activity of cucumber under blue light is the highest,in cucumber the photosynthetic capacity of the strongest is under green,plants fill light in red and blue mixed make green light will obvious improve the capacity of the plant photosynthesis.It is usable for QE6500 fiber spectrometer in the determination of chlorophyll fluorescence.
出处
《中国农学通报》
CSCD
北大核心
2011年第10期161-167,共7页
Chinese Agricultural Science Bulletin
基金
国家科技部"十五"国家科技攻关计划"冀东秋黄瓜品种遗传改良及优质栽培配套技术研究"(2004BA525B14)
河北省科技支撑计划"蔬菜无公害优质高效生产关键技术研究"(07220701D)
关键词
光质
白黄瓜
叶绿素荧光
单色光
混合光
light qualities
white cucumber
chlorophyll fluorescence
single light
mixed lights
