摘要
利用抗坏血酸对铁（ Ⅲ）２〔２（６甲基苯并噻唑） 偶氮〕５二乙氨基苯甲酸络合物的还原作用，建立了一个间接光度测定抗坏血酸的方法． 在ｐＨ５０ ～７０ 的ＨＡｃＮａＡｃ 的缓冲介质中， 络合物的最大吸收峰位于６３５ ｎｍ ， 摩尔吸光系数达到２２ ×１０５Ｌ·ｍｏｌ－ １·ｃｍ －１ ． 抗坏血酸浓度在０ ～０６４ μｇ／ ｍＬ范围内与吸光度呈良好的线性关系． 方法适用于市售维生素Ｃ片剂和针剂中抗坏血酸的测定，
A new sensitive determination procedure for ascorbic acid was developed based on the reduction of ascorbic acid to Fe(Ⅲ) 2 〔2 (6 methylbenzothiazoly)azo〕 5 diethylaminobenzoic acid complex.In the presence of HAc NaAc buffer(pH=5 8)and SDS,the maximum absorption wavelength of the system is 635?nm and absorptivity is 2 2×10 5L·mol -1 ·cm -1 .The absorbance is directly proportional the concentration of ascorbic acid in the range of 0～0 64?μg/mL.And the proposed method was used to determine the concentration of ascorbic acid in pharmaceuticals.
出处
《西北师范大学学报（自然科学版）》
CAS
1999年第4期49-51,共3页
Journal of Northwest Normal University(Natural Science)
关键词
间接分光光度法
维生素C
药物
测定
BTAEB
ascorbic acid
indirect spectrophotometry
2 〔2 (6 methylbenzothiazoly)azo〕 5 diethylaminobenzoic acid
