摘要
细胞内的RNA一般不会单独存在,而是与各种各样的RNA结合蛋白(RBPs)绑定在一起,形成核糖核蛋白复合体(RNP complexes)影响着RNA的加工与转归.Poly(C)-结合蛋白是一类重要的RNA结合蛋白,可分为两组:hnRNP K和PCBP1-4.它们以序列特异的方式与核酸嘧啶富含区相结合.这类蛋白具有共同的结构模体(motif),即hnRNP K同源(KH)域.KH域是与mRNA结合的结构基础,也是机体内调控系统的组成部分,可使得Poly(C)-结合蛋白参与蛋白/核酸、蛋白/蛋白之间的相互作用,范围涉及复制、转录、mRNA稳定和翻译控制过程等.对Poly(C)-结合蛋白功能的深刻认识可使我们洞察多种疾病的病理生理过程.
The processing and ultimately turnover of mRNA are complex processes that are highly regulated.Within a cell,RNA is usually not in a naked form.It forms ribonucleoprotein（RNP） complexes with various RNA-binding proteins（RBPs）,thereby influencing processing and turnover events.Poly（C）-binding proteins（PCBPs）,generally known as RBPs,interact in a sequence-specific fashion with single-stranded poly（C）.They can be divided into two groups： hnRNP K and PCBP1-4.PCBPs and hnRNP K share a common structural motif,the hnRNP K homology（KH） domain,which provides a structural basis for mRNA binding.The KH-domains are components of a modular system,which enables the proteins to be engaged in both protein /nucleic acid and protein /protein interactions.The latter interactions are involved in cell signaling events.As components of different mRNA-protein complexes,PCBPs and hnRNP K have been identified as factors involved in regulating duplication,transcription,mRNA stability and translation.They have also been found related to some pathological processes,such as Hutchinson-Gilford Progeria syndrome,virus infections and malignancy.
出处
《中国生物化学与分子生物学报》
CAS
CSCD
北大核心
2011年第3期205-211,共7页
Chinese Journal of Biochemistry and Molecular Biology
基金
国家重点基础研究发展规划(973计划)资助项目(No.2006CB500700)~~
