摘要
为探索银杏液体悬浮细胞培养条件下产生黄酮的工艺条件,以银杏液体悬浮系为试验材料,在250 mL的三角瓶中装入80 mL液体培养基,置于25℃,100 r/min转速下摇床培养,试验设计分别为光照与黑暗、培养时间、细胞聚集体的大小、前体物对银杏液体悬浮细胞系产生黄酮的影响。结果表明:光照条件下培养14 d黄酮含量最高,3～4 mm粒径的细胞聚集体是黄酮合成的适宜细胞聚集体,前体物L-苯丙氨酸和乙酸钠对黄酮合成无明显的促进作用,且对细胞生长有抑制作用。结论:初步探索到银杏液体悬浮细胞培养条件下产生黄酮的工艺条件,为银杏细胞悬浮培养工业化生产黄酮类物质提供一定的理论基础和技术参数。
Objective：Conditions for producing flavonoids were studied by the cell suspension culture of ginkgo.Methods： In cell suspension cultures,the suspension was placed in 80 mL of liquid medium in a 250 ml-flask and kept on reciprocal shaker（100 r/min） at 25 ℃.The conditions of suspension cultures detected were as follows： light/darkness,culture time,size of cell aggregates and content of precursors.Results： The production of flavonoid reached a maximum by culture for 14 days under light.The cell aggregates with 3~4 mm of diameter were suitable for flavonoids synthesis.L-phenylalanine and sodium acetate as precursors had no significant promotion on flavonoids synthesis,however the cell growth was inhibited.Conclusion： Conditions for producing flavonoids were obtained by the cell suspension culture of ginkgo,which will further optimize the culture conditions required for the industrial production of flavonoids by the cell suspension culture of ginkgo.
出处
《江西农业大学学报》
CAS
CSCD
北大核心
2011年第2期360-363,374,共5页
Acta Agriculturae Universitatis Jiangxiensis
基金
武汉市高校科学研究资助项目(2008K079)
湖北省教育厅科学技术研究项目(B20094008)
