摘要
以戊二醛为交联剂,壳聚糖为载体固定化粪产碱杆菌来源的青霉素G酰化酶.通过单因素和正交试验优化确定固定化最佳条件:0.1 g载体,2.5%戊二醛,酶量160 U,0.6mol/L NaCl,0.2 mol/L磷酸缓冲液9.5 mL3,7℃,pH 8.0,固定化38 h.固定化酶最高比活为48.7 U/g湿载体.固定化酶性质研究表明其最适反应温度为55℃,最适pH 9.5;具有较好的批次反应稳定性.
Penicillin G acylase from Alcaligenes faecalis is immobilized with glutaraldehyde cross-linked chitosan.By the optimization of the immobilized conditions using single factor and orthogonal experiment,the optimum conditions for the enzyme immobilization obtained are as follows： 0.1 g carrier,160 U of enzyme,the final concentration of glutaraldehyde is 2.5%（g/v）,phosphate buffer（pH 8.0,0.2 mol/L） 9.5 mL,0.6 mol/L NaCl,temperature 37 ℃,and the immobilized time is 38 h.The highest activity of the immobilized enzyme is 48.7 U/g wet carriers,and the optimum temperature and pH value of the immobilized enzyme are 55 ℃ and 9.5,respectively.Furthermore,the batch cycle numbers show the stable property of the immibilized enzyme in the conversion of penicillin G to 6-aminopenicillanic acid.
出处
《烟台大学学报（自然科学与工程版）》
CAS
北大核心
2011年第2期163-166,共4页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
鲁东大学科研基金(LY20083305)
关键词
壳聚糖
戊二醛
固定化
青霉素G酰化酶
chitosan
glutaraldehyde
immobilized
penicillin G acylase
