摘要
目的研究急性髓系白血病(AML)中WNT信号传导通路拮抗基因分泌型卷曲相关蛋白5(SFRP5)基因的甲基化状态。方法应用甲基化特异性PCR(MSP)法对99例AML患者的骨髓或外周血进行SFRP5基因启动子区甲基化状况检测,并以70例门诊普通病人的外周血作为对照。结果 99例AML患者中检出10例(10.1%)SFRP5的甲基化,70例正常对照中仅检出1例(1.4%)SFRP5的甲基化。SFRP5在AML患者中的甲基化率显著高于正常对照(P<0.05)。SFRP5基因的甲基化状态与年龄、性别无关(P>0.05),与AML的临床分型相关(P<0.05)。结论 SFRP5基因的甲基化与AML有相关性,SFRP5基因的甲基化可能是引起AML的分子机制之一。
Objective To investigate the methylation status in the promoter region of secreted frizzled related protein 5 （SFRP5） gene in acute myeloid leukemia （AML）. Methods MSP method was applied to examine the promoter methylation of SFRP5 gene in 99 bone marrow or peripheral blood samples of AML patients. As controls, 70 normal peripheral blood samples from volunteers of general outpatients were examined. Results All 113 samples were involved in the results analysis. In 99 patients of AML, 10 samples（ 10. 1% ） showed SFRP5 gene methylation. In 70 controls, 1 sample（ 1.4% ） showed SFRP5 gene methylation. And all of them were semi - methylation status. The frequency of SFRP5 gene methylation in AML patients was significantly higher than that in controls （P 〈 0. 05 ）. There was no significant difference between the different age groups and gender for the results of the methylation status in AML（ P 〉 0. 05）. The methylation status of SFRP5 gene was associated with the clinical classification of AML （P 〈 0. 05）. Conclusion The methylation status of SFRP5 gene was correlated with AML. The methylation of SFRP5 gene may be one of the molecular mechanisms of AML.
出处
《医学研究杂志》
2011年第3期25-28,共4页
Journal of Medical Research
基金
国家自然科学基金资助项目(30972709)
卫生部北京医院重点基金资助项目(BJ-2008-76)
