摘要
目的通过对组织芯片和普通切片的比较,探讨组织芯片技术在免疫组化实验中的有效性。方法选择84例人结直肠癌标本蜡块,分别制作普通切片和组织芯片,采用免疫组化技术检测促凋亡基因（Bax）、谷胱甘肽-S-转移酶（GST-π）、耐药蛋白（LRP）、P-糖蛋白（P-gp）和DNA拓扑酶Ⅱ（TopoⅡ）的表达。结果组织芯片与普通切片Bax、GST-π、LRP、P-gp和TopoⅡ的表达呈显著正相关（r=0.795-0.867,P〈0.001）,两者Bax、GST-π、LRP、P-gp及TopoⅡ的表达一致率分别达90.24%、93.90%、91.46%、90.24%、92.68%。组织芯片与普通切片Bax、GST-π、LRP、P-gp和TopoⅡ表达比较,差异无统计学意义（χ2=0.125~1.500,P〉0.05）。结论采用规范的组织芯片制作程序,2 mm直径的组织芯片在进行免疫组化染色时能够代表普通切片。该技术是一种可靠的、有代表性的、有效的高通量组织分析工具。
Objective To evaluate the effectivity of tissue microarrays technology in immunohistichemical experiment by comparing tissue microarrays with ordinary section. Methods Paraffin block specimens of colorectal cancer of 84 patients were selected to make tissue microarrays and ordinary sections.Expressions of Bax,GST-π,LRP,P-gp and Topo Ⅱ were detected immunohistochemically. Results A significantly positive association was observed between the expressions of ordinary section and the tissue microarrays（r=0.795-0.867,P〈0.001）.Concordance of expressions between the ordinary section and the tissue microarrays was 90.24% for Bax,93.90% for GST-π,91.46% for LRP,90.24% for P-gp,and 92.68% for Topo Ⅱ,respectively.The discordance of expressions between the ordinary section and tissue microarrays was not significant（χ2=0.125-1.500,P〉0.05）. Conclusion Employing a normative tissue microarrays-making procedure,the array of 2 mm in diameter can replace the common section in immunohistochemical staining,which is a reliable,typical or effective high-flux tool for tissue analysis.
出处
《齐鲁医学杂志》
2011年第1期22-24,共3页
Medical Journal of Qilu
关键词
结直肠肿瘤
微阵列分析
免疫组织化学
colorectal neoplasms
microarray analysis
immunohistochemistry
