摘要
利用啤酒糟为培养基对黑曲霉固态发酵产β-葡萄糖苷酶的工艺条件进行了优化和动力学研究。单因素试验表明,最适产酶温度、料液比和接种量分别为30℃、1∶5(啤酒糟∶水,g∶mL)和10%(mL/g);利用L9(34)正交试验优化反应条件,结果表明,在25℃,初始料水比为1:5,接种量10%的条件下,培养4d,β-葡萄糖苷酶的酶活可达10.85U/g。动力学研究表明,β-葡萄糖苷酶在96h进入产酶的高峰期,120h达到酶活最大值。
The solid fermentation condition of β-glucosidase production was optimized by Aspergillus niger with only brewer′s grain as medium,and the fermentation dynamics was studied as well.The single-factor test showed the optimum temperature,ratio of medium-liquid and inoculum volume were 30℃,1：5（brewer′s grain： water,g：mL） and 10%（mL/g）,respectively.The result of L9（34） orthogonal test showed that the activity of β-glucosidase reached 10.85 U/g after 4 days at 25℃,inoculum volume 10%,and ratio of brewer′s grain to liquid 1：5.The highest yield of β-glucosidase activity was obtained after 120h.
出处
《生物学杂志》
CAS
CSCD
2011年第2期51-53,共3页
Journal of Biology
基金
安徽省教育厅自然科学一般项目(KJ2009B155)
蚌埠学院自然科学一般项目(2010ZR09)
关键词
啤酒糟
黑曲霉
Β-葡萄糖苷酶
发酵
brewer′s grain
Aspergillus niger
β-glucosidase
fermentation
