摘要
采集上海及周边地区猪内脏样本,进行猪圆环病毒2型（Porcine circovirus type 2,PCV-2）病原学检测,并对阳性样本的ORF2序列进行分析和比对。结果显示,发病猪群PCV-2阳性率为39.06%（25/64）,健康猪群阳性率为13.51%（5/37）,PCV-2总阳性率为29.70%（30/101）。30份阳性样本中PCV-2a亚型为2株,检出率为1.98%（2/101）,其余28株均为PCV-2b亚型,检出率为27.72%（28/101）。PCV-2的ORF2序列与已登录的ORF2序列的同源性在90.7%~100%。2株PCV-2a株相互间的同源性为99.6%;28株PCV-2b株的同源性在94.4%~100%,PCV-2b株中只有4株与已登录株DQ141322、AY321984和AY484413株同源性关系最近,处于同一分支中,而另外24株与Q151643中国株同源性关系最近且处于同一分支中。研究结果表明上海及周边地区PCV-2感染比较普遍,发病猪场的感染率远高于健康猪场,且感染率比往年有升高,PCV-2在猪场疫病的爆发和传染中起着重要的协同作用;在PCV-2的感染病例中,PCV-2b亚型为感染的优势毒株;绝大多数PCV-2b ORF2片段的同源性与已登录的一株中国株同源性关系密切,在基因进化树上处于同一分支,但是否可以推断其为PCV-2的另一个亚型有待进一步考证。
Porcine circovirus type 2 was detected in porcine tissue samples from Shanghai and neighboring area.The positive percentage of PCV-2 was 39.06%（25/64） in sick pigs,13.51%（5/37） in healthy pigs,and 29.70%（30/101） in all samples.The virus isolation rates of PCV-2a genotype and PCV-2b genotype were 1.98 % and 27.72%,respectively.Sequence analysis illustrated that nucleotide homologies of ORF2 of PCV-2a and PCV-2b isolates were 99.6% and 84.4%-100%,respectively.Out of 28 PCV-2b isolates,24 isolates belong to one group represented by Q151643.This study revealed that in Shanghai and neighboring area,PCV-2 infection was highly prevalent,and infection rate in sick pigs was much higher than in healthy pigs.The PCV-2b group strains were predominant strain.
出处
《中国动物传染病学报》
CAS
2011年第1期27-30,共4页
Chinese Journal of Animal Infectious Diseases
关键词
猪圆环病毒2型
基因型
分析
Porcine circovirus type 2
genetype
analysis
