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The effect of Tagalsin on mice with transplanted H22 Hepatocarcinoma

The effect of Tagalsin on mice with transplanted H22 Hepatocarcinoma
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摘要 Objective: The aim of our study was to explore the inhibitory effect of Tagalsin on murine transplanted tumour and its anti-tumour mechanisms. Methods: Animal models were established by transplanting H22 hepatoma cells to the left oxter of mice, and ten days later they were randomly divided into five groups: blank control group (edible oil), positive control group (HCFU) and Tagalsin group, including low-dose, middle-dose and high-dose group. All mice were killed 24 h after medication, during which observation was conducted concerning survival conditions, body weight changes, spleen weight and tumor weight of tumor-bearing mice; the spleen index and the tumor inhibitor rate (IR) were calculated and pathological changes of tumor-bearing mice were observed by HE dye. Apoptosis factors p53 and Survivin mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR). Results: Tagalsin can inhibit hepatoma growth effectively without influencing spleen index and body weight, the tumor inhibitor rate (IR) of low, middle and high dose group of Tagalsin were 15.81%, 36.75% and 74.79% respectively, the tumor inhibitor rate (IR) of HCFU were 73.93%. Apoptosis cells could be found from the specimen of the positive control group and Tagalsin groups. Reverse transcription polymerase chain reaction (RT-PCR) results showed that positive control group’s and Tagalsin treatment groups’ p53 gene expression enhanced significantly and Survivin gene expression dropped comparing with blank group (P 0.05). Conclusion: Tagalsin can inhibit growth of the H22 hepatoma cells significantly, the mechanism of anti-tumor effect may work by up-regulating p53 expression and down-regulating Survivin expression. Tagalsin may be considered as a potential candidate for chemoprevention.
出处 《The Chinese-German Journal of Clinical Oncology》 CAS 2011年第3期153-156,共4页 中德临床肿瘤学杂志(英文版)
基金 Supported by grants from the National "863" Scientific Item (No.2006AA09Z446) Science and Technology of Qingdao (No. 07-2-1-8-NSH-1)
关键词 Tagalsin H22 RT-PCR SURVIVIN p53 荷瘤小鼠 肝癌 移植效果 Survivin 逆转录聚合酶链反应 抗肿瘤机制 p53基因 体重变化
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