摘要
目的:研究贞芪扶正胶囊对大鼠肝细胞色素P450酶CYP1A2、CYP3A4、CYP2E1的影响。方法:用Cocktail探针药物法,将Wistar大鼠按体重随机分组,灌胃给予贞芪扶正胶囊溶液,以生理盐水为空白对照,诱导10天,股动脉插管,注射给予Cocktail探针药物咖啡因、氨苯砜、氯唑沙宗,通过HPLC检测各探针药物的代谢率来评价各组的CYP1A2、CYP3A4、CYP2E1亚型酶的活性;药动学计算采用DAS2.0软件完成。结果:给予贞芪扶正胶囊组大鼠CYP2E1的酶活性明显低于对照组,探针药物氯唑沙宗的t1/2显著延长;CYP1A2、CYP3A4的的水平没有显著变化。结论:贞芪扶正胶囊对大鼠CYP2E1有抑制作用,对大鼠CYP1A2、CYP3A4的影响不显著。
Objective： To study on the effect of Zhenqi Fuzheng Capsules to cytochrome P450 isozymes CYP1A2、CYP3A4、CYP2E1.Methods： Using methods of Cocktail probe drugs.The rats were tandomly divided into two groups.one group were given Zhenqi Fuzheng once daily orally for ten days.Another group received orally normal saline one daily as the blank control.After ten days,after femoral catheterization,the rats were given probe drugs of coffine,dapsone and chlorzoxazone.The plasma concentration of probe drugs were determined by HPLC,Data of plasma drug level-time were disposed with DAS Ver 2.0.Results： The CYP2E1 after received Zhenqi Fuzheng was reduced,but the CYP3A4 and CYP2E1 contents were not markedly changed.Conclusion： It suggested that Zhenqi Fuzheng tended to be the inhibitor of the CYP2E1,the CYP3A4 and CYP2E1 were hardly affected.
出处
《中药药理与临床》
CAS
CSCD
北大核心
2011年第1期84-86,共3页
Pharmacology and Clinics of Chinese Materia Medica
