摘要
目的探讨大气颗粒物PM10和PM2.5对人肺成纤维细胞及其分泌肿瘤坏死因子(TNF-α)、白细胞介素6(IL-6)和白细胞介素8(IL-8)的影响。方法于冬季采暖期采集颗粒物样品PM10和PM2.5,实验细胞为传代培养的人肺成纤维细胞,用剂量分别为25、50、100、200μg/ml的PM10和PM2.5对人肺成纤维细胞染毒24 h。采用四甲基偶氮唑盐微量酶反应比色法测定细胞毒性;放射免疫法测定炎性因子TNF-α、IL-6和IL-8。结果 25、50、100、200μg/ml的PM10作用于人肺成纤维细胞24 h后产生一定的毒性作用,低剂量时刺激细胞增殖,高剂量时抑制细胞增殖,各浓度时细胞存活率分别为118.80%,120.47%,107.42%,95.97%。25、50、100、200μg/ml的PM2.5作用24 h后亦对人肺成纤维细胞产生一定的毒性作用,亦表现为低剂量时刺激细胞增殖,高剂量时抑制细胞增殖,各浓度时细胞存活率分别为107.81%,101.48%,91.86%,81.35%。PM10和PM2.5能刺激人肺成纤维细胞分泌炎性因子TNF-α、IL-6和IL-8,尤其200μg/ml浓度时与对照组相比,各炎性分子浓度差异有统计学意义(P<0.05)。结论大气颗粒物PM10和PM2.5对人肺成纤维细胞有一定毒性作用;PM10和PM2.5染毒24 h后能诱导人肺成纤维细胞分泌炎性因子TNF-α、IL-6、IL-8。
Objective To study the effects of PM10 and PM2.5 on the secretion of tumor necrosis factor-α （TNF-a）, interleukin-6 （IL-6） and interleukin-8 （IL-8） in the human lung fibroblast （HLF） in vitro. Methods PM10 and PM2.5 were collected during heating period. The tested cells were passage cultured HLF. Cytotoxicity of the particles was measured by MTY assay. The levels of cytokines TNF-α,IL-6 and IL-8 were determined by using radioimmunity assay. Results After 24h of treatment, PM10 and PM2.5 caused cytotoxicity to HLF, and the survival rate of the cells increased with exposure of PM10 and PM2.5 increased at low level, but decreased with exposure of PM10 and PM2.5 increased at high level. PM10 and PM2.5 increased the secretion of TNF-α, IL-6 and IL-8 in HLF. Conclusion PM10 and PM2.5 exposure my cause cytotoxicity to HLF： After 24 h of treatment. PM10 and PM2.5 can induce the early inflammatory reaction.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2011年第3期206-208,共3页
Journal of Environment and Health
基金
北京市自然科学基金(7042009)
关键词
颗粒物
人肺成纤维细胞
炎性因子
Particulate
Human lung fibroblast
Inflammatory factor
