摘要
根据GenBank上登录的果胶酶基因保守序列设计引物,从本实验室已筛选的一株具有降解果胶质功能的枯草芽孢杆菌S-1中克隆到了pelB基因片段,pelB与pMD20-T载体连接后转化到大肠杆菌JM109中,测序并构建进化树分析。结果表明,其序列与来源于Bacillus sp.果胶裂解酶pel-15和pelA的同源性分别为40.98%和39.20%;与Bacillus pumilus的pelB一致性为40.32%。推断此pelB为类似果胶酶基因片段。
A pair of primers was designed according to the conservative sequence of the reported pectinase gene in GenBank.The fragment of pelB gene,amplified from Bacillus subtilis S-1 which has the ability to degrade pectin,was cloned into pMD20-T vector and then transformed into the competent E.coli JM109 cells.The sequence of pelB gene was analyzed by NJ phylogenetic tree.Results indicated that pelB gene was similar to pectate lyase.And pelB gene showed sequence similarity with pectate lyase of Bacillus sp.（40.98% sequence identity with pel-15 and 39.20% sequence identity with pelA）,and 40.32% sequence identity with pelB of Bacillus pumilus.
出处
《生物技术通报》
CAS
CSCD
北大核心
2011年第5期209-212,共4页
Biotechnology Bulletin
