摘要
RNA干扰(RNAinterference,RNAi)是一种由双链RNA(double-stranded RNA,dsRNA)介导、能够特异沉默靶基因的转录后基因沉默现象,为植物基因功能的研究开辟了新途径。本研究主要综述了RNA干扰现象的发现、RNA干扰的过程及其特点、RNA干扰在植物中的诱导方法及载体构建、近年来RNA干扰在植物基因功能和抗病性等方面的研究进展,以及对该技术的理论研究和应用展望,以期对RNA干扰技术在植物中的深入研究提供指导。
RNA interference （RNAi） is a phenomenon of post-transcription target gene silencing in organisms, in whcih the gene was silenced triggering by double-stranded RNA （dsRNA）, and it is a new approach to conduct a research on gene function. The paper reviewed the discovery of RNAi phenomenon, its interference function and characters, induction methods and vector construction of the interference in plants, and research proceedings of gene functions and disease-resistance by RNAi, and future development of its theory and application, which would provide some guidance for further studies.
出处
《草业科学》
CAS
CSCD
北大核心
2011年第5期823-830,共8页
Pratacultural Science
基金
国家自然科学基金(30771527)
