摘要
目的 检测人类成骨样细胞MG-63中甲状腺激素受体（TR）的表达情况以及甲状腺激素（T3或T4）对其的影响.方法 用实时荧光定量PCR法对MG-63细胞中TR的主要4个亚型,α1、α1、β1、β2的mRNA表达进行了测定.结果 TRα1 mRNA的表达水平最高,为10.70±0.45;TRβ1仅及其半,为5.75±0.10;TRβ2不低,相当于TRβ1的60%,为3.34±0.08;TRα2的表达极低,仅为（3.66±0.59）×10-2.分别加入不同浓度的T3或T4,仅TRα1和TRα2对T3表现明显的降调节,且TRα1和TRα2的表达与相应T3浓度之间负相关,其他亚型无显著性变化,也无相关关系;同样浓度范围的T4作用各亚型,表达均无显著性变化.结论 在骨细胞发育分化中TRα1起关键作用.
Objective To examine the expression of thyroid hormone receptor isoforms （TR α1, α2, β1, and β2） in human osteoblast-like cell line MG-63 at the mRNA level and the effect of thyroid hormone （T3 or T4 ） on the expression. Methods Realtime quantitative PCR was performed. Results The expression of TRα1 mRNA was the highest, that was 10. 70± 0.45, TRβ1 was 5.75 ± 0. 10, TRβ2 was 3.34 ± 0. 08, and TRα2 was very low, only （3.66 ±0. 59） × 10-2. Only the expression of TRαl and TRα2 mRNA was down regulated significantly by the treatment of 10-10 ～ 10-6 mol/L T3, and there was a negative correlation between the expression of TRα1 or TRα2 mRNA and the concentration of T3. Conclusion TRα1 plays a primary role in mediating the effects of thyroid hormones in skeletal development.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2011年第3期242-245,共4页
Chinese Journal of Endocrinology and Metabolism
基金
国家自然科学基金资助项目（30271154）
