摘要
以嗜水气单胞菌为实验对象,研究了表没食子儿茶素没食子酸酯(EGCG)对其的抑菌活性及抑菌机制。结果表明,EGCG对嗜水气单胞菌的最小抑菌浓度均为25～50μg/mL。菌体在EGCG作用下,改变细胞膜的通透性,电导率增大,细胞内物质渗漏,从而影响细胞代谢,最终导致细胞死亡。
Antimicrobial effect of EGCG against Gram-negative Aeromonas hydrophila was investigated.As a result,minimal inhibitory concentration（MIC）of EGCG against Aeromonas hydrophila was 25～50μg/mL.The mechanism of action was further investigated by cell integrity and membrane permeabilization assays.The assay showed that EGCG obviously increased the membrane permeability,and induced the leakage of ion and macromolecules in the cytoplasm.Flow cytometric analysis revealed that EGCG can cause significant membrane damage for influx of ONPG into cells.Results indicated that EGCG killed Aeromonas hydrophila by disrupting the structure of cell membranes and caused the leakage of intracellular components from bacterial cells.
出处
《食品工业科技》
CAS
CSCD
北大核心
2011年第5期98-101,104,共5页
Science and Technology of Food Industry
基金
国家科技部中小企业技术创新基金项目(07C26115201947)
农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放课题(BZ2009-24)
关键词
EGCG
嗜水气单胞菌
抑菌机理
EGCG
Aeromonas hydrophila
antimicrobial mechanism
