摘要
从农药厂排污沟污泥中分离到一株能降解毒死蜱的新菌株，命名为R17，经生理生化和16SrDNA序列同源性分析，鉴定为Sphingopyxis terrae R17可以利用毒死蜱作为唯一碳源生长，该菌株的最适生长温度为35℃、最适pH为7～8，在此条件下培养28h后，菌落浓度达9．18×10^8cfu／mL。研究了该菌株在不同时间，对不同浓度毒死蜱的降解特性，结果：羡明，当接菌量为9．18×10^9cfu／mL时，在1d、2d、3d和4d内对10mg／L的毒死蜱降解率分别达到18．59％、31．23％、36．55％和47．69％以上；在2d内对1mg／L、5mg／L和10mg／L的毒死蜱降解率分别达到50．21％、43．46％和31．23％。
One bacterial strain, named R17, was isolated from the sludge polluted with chlorpyrifos which had the capability to utilize chlorpyrifos as the sole carbon and energy sources. Strain R17 was identified preliminarily as Sphingopyxis terrae based on its physiological and biochemical characters and its 16S rDNA homologue sequence analysis. Under the optimal growth condition, 35 ℃and pH 7 - 8. Strain R17 could reach 9.18× 10^8 cfu/mL after 28 h. The chlorpyrifos degradation characteristics of strain R17 were studied with different times and different initial chlorpyrifos concentrations. The results showed that with the inoculation of 9. 18 × 10^7 cfu/mL, the degradation rate of chlorpyrifos by strain R17 were 18.59 % , 31.23 % , 36.55 % and 47.69 % in 1 d, 2 d, 3 d and4 d respectively. The degradation rate of R17 with initial concentration of 1 mg/L, 5 mg/L, 10 mg/L chlorpyrifos reached 50.21% , 43.46 % and 31.23 % after 2 d, respectively.
出处
《激光生物学报》
CAS
CSCD
2011年第2期261-268,共8页
Acta Laser Biology Sinica
基金
国家"863"高技术研究发展计划项目(2007AA102403)
安徽省科技攻关项目(08010302166)
国家科技支撑计划项目(2007BAD87B06)
