摘要
目的探讨以PNA作为杂交探针与普通的DNA探针相比,在漏声表面波生物传感器生物杂交反应中的优越性。方法用巯基固定法将PNA、DNA两种探针分别固定在漏声表面波生物传感器的金膜表面,分别与完全匹配、1个及2个碱基错配的靶序列进行杂交反应,观察两种不同的探针与靶序列反应所引起的相位变化及反应平衡时间。结果与DNA探针相比,PNA探针识别碱基错配的能力显著提高,且杂交平衡时间更短。结论 PNA探针可提高漏声表面波生物传感器的检测特异性。
OBJECTIVE To investigate the superiority of the biological hybridize reaction of the leakage of surface acoustic wave（LSAW） biosensor by comparing the probe of PNA with the probe of DNA.METHODS The probe of PNA and DNA were immobilized on the gold surface with thiol immobilization method,and hybridized reaction with target sequence of the complete,one mismatch and two mismatch sequence,respectively.The change of the phase shift and the equilibrium time of the reaction of the two different probes were observed.RESULTS The recognizing mismatch base capability of PNA probe was significantly improved and the equilibrium time of hybridization was shorter comparing with the probe of DNA.CONCLUSION PNA as the probe may improve the determined specificity of LSAW biosensor.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2011年第10期1980-1983,共4页
Chinese Journal of Nosocomiology
基金
国家自然科学基金(81000776
30927002)
国家传染病重大专项分题(2008ZX10003-02)
重庆市自然科学基金(CSTC
2010BB5194)
全军医药卫生科研基金(06G073
08G089)
国家计生委课题(2009-GJKJS-017-019)
第三军医大学青年人才创新基金(2010XQN26)
关键词
漏声表面波
肽核酸
传感器
特异性
Leaky surface acoustic wave
Peptide nucleic acid
Sensor
Specificity
