摘要
为了解在PRRSV持续性感染猪群中的抗体动态,对蓝耳病阳性猪场育肥猪、经产母猪、后备母猪及仔猪每周采血1次,分离血清,用RT-PCR方法检测PRRSV,ELISA试剂盒检测抗PRRSV抗体并检测猪瘟疫苗免疫后的抗体水平。结果表明,在猪群康复后PRRS抗体水平呈下降趋势,并逐渐趋向于稳定,当整个猪群再次表现临床症状时,抗体逐渐上升至一定水平,但抗原检出率较低;猪瘟疫苗免疫后抗体水平上升依然很快,免疫效果较好。
In order to study the dynamics of the antibody of PRRSV and CSFV in the swinery with persistent infection of PRRSV,the blood samples of finishing pigs,multiparous sow,replacement gilts and piglets in pig farm with PRRS were collected once a week.PRRSV were tested by RT-PCR,and the antibodies of PRRSV and HCV were tested by ELISA after vaccination of CSFV vaccine.The results indicated that the level of PRRSV antibody went down and remained stable gradually after swine herds recovered,and rose gradually to higher but the detection rate of PRRSV was comparatively lower when the entire swine herds showed clinical symptoms once again.The level of HCV antibody rose quickly after immunized by classical swine fever vaccine and the immunity effect was better.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2011年第5期630-632,共3页
Chinese Journal of Veterinary Science
基金
山东省自主创新成果转化重大专项计划资助项目(2008ZHZX1A1101)
