摘要
目的探讨巨噬细胞移动抑制因子(MIF)在缺氧缺血性脑损伤(HIBD)新生大鼠中的作用及可能机制。方法健康7日龄新生SD大鼠共120只。随机分为3组,分别为假手术组、HIBD组、抗MIF中和抗体干预组(anti-MIF组,缺氧缺血后即予抗MIF中和抗体5 mg.kg-1腹腔注射),每组40只。于HIBD模型制成后6 h、12 h、24 h,3 d及7 d处死,应用免疫组织化学法(SP法)观察各组脑缺血侧皮质区MIF及核因子-κB(NF-κB)蛋白的表达,双抗夹心酶联免疫吸附法(ELISA)测定各组缺血侧脑组织匀浆TNF-α水平。结果 HIBD组新生大鼠脑组织皮质区MIF、TNF-α表达均在缺氧缺血6 h明显增加,24 h达高峰(Pa<0.01),7 d时恢复正常,与假手术组比较差异无统计学意义(P>0.05);HIBD组NF-κB表达在缺氧缺血6 h开始增加,24 h达高峰,并维持至3 d(Pa<0.01),7 d降至正常,与假手术组比较差异无统计学意义(P>0.05);anti-MIF组大鼠脑组织MIF、NF-κB、TNF-α的表达与HIBD组比较,在各个实验时间点均有降低(Pa<0.01)。结论 MIF可能通过调控NF-κB的途径影响其脑皮质TNF-α水平,从而介导缺氧缺血后新生大鼠脑组织的炎性损伤。
Objective To explore the role and pathomechanism of macrophage migration inhibitory factor（MIF） in neonatal rats with hypoxic-ischemic brain damage（HIBD）.Methods Seven-day newborn SD rats were randomly divided into 3 groups：sham operation group（n=40）,HIBD group（n=40） and intervention group with anti-MIF（n=40,5 mg·kg-1,intraperitoneal injection）.The rats were sacrificed at 6 h,12 h,24 h,3 d and 7 d after hypoxic-ischemic（HI）,and brain tissues were collected,immunohistochemistry was used to detect the expression of MIF and nuclear factor-kappa B（NF-κB） protein in the pallium,and the content of TNF-α in ischemic cerebral brain was detected by enzyme-linked immunosorbent assay（ELISA）.Results Compared to the sham control group,the expression of MIF and TNF-α in palliumal cells in HIBD group were significantly increased at 6 hours after HI,peaked at 24 hours（Pa0.01）,but no significant difference at 7 days（P0.05）;The expression of NF-κB increased at 6 hours after HI,peaked at 24 hours and maintained until the 3rd day（Pa0.01）,but no signifficant difference at 7 days（P0.05）.However the concentrations of MIF,NF-κB,TNF-α were significantly down-regulated in anti-MIF intervention group compared with those in HIBD group（Pa0.01）.Conclusion Perhaps MIF is involved in the immune reaction of HIBD by controlling NF-κB pathway that can change the content of TNF-α.
出处
《实用儿科临床杂志》
CAS
CSCD
北大核心
2011年第8期609-612,共4页
Journal of Applied Clinical Pediatrics
关键词
巨噬细胞移动抑制因子
脑损伤
缺氧缺血
大鼠
新生
macrophage migration inhibitory factor
brain damage
hypoxic-ischemic
neonatal rat
