摘要
目的:观察红景天苷(salidroside,Sal)对老龄大鼠局灶性脑缺血再灌注损伤的保护作用并探讨其作用机制。方法:采用线栓法制备老龄大鼠局灶性脑缺血再灌注损伤模型,观察红景天苷对老龄大鼠局灶性脑缺血再灌注后神经功能障碍,脑水肿及脑梗死范围以及对脑缺血2h再灌注24h缺血脑组织能量物质ATP、葡萄糖无氧酵解产物以及体内自由基清除剂SOD、GSH和脂质过氧化产物MDA等生化物质的影响。结果:脑缺血2h再灌注24h腹腔注射Sal40,20,10mg.kg-1可明显减轻神经功能障碍(P<0.05,P<0.01),减轻脑水肿(P<0.05,P<0.01)及缩小脑梗死范围(P<0.05)。同时Sal40,20,10mg.kg-1组与缺血再灌注组相比,缺血脑组织内SOD活性和GSH活力明显增高(P<0.05,P<0.01),MDA含量显著降低(P<0.05,P<0.01),大鼠脑组织内ATP含量显著升高(P<0.05,P<0.01),脑组织内LA含量明显降低(P<0.05,P<0.01)。结论:红景天苷对老龄大鼠局灶性脑缺血再灌注损伤有良好的保护作用。其机制与其改善缺血脑组织能量代谢、降低自由基损伤有关。
OBJECTIVE To study the protective effects of salidroside（Sal） in aged rats with cerebral ischemia-reperfusion in- jury and its mechanism of action. METHODS Right middle cerebral artery was occluded by inserting a thread through internal carotid artery for 2h, and then reperfused for 24h in rats. The experiment observed the protective effects of Salidroside on nerve dysfunction,cerebral edema,the range of cerebral infarction in aged rats. The activity of superoxide dismutase（SOD）,contents of reduced glutathione（GSH）, malondialdehyde（MDA）, adenosine 5-triphosphorate（ATP）, and lactate acid（LA） in experimen- tal aged rats＇brain were measured after focal brain ischemia-reperfusion injury. RESULTS Salidroside which was infected peri- toneally at the doses of 40 mg.kg-1 ,20 mg, kg 1 and 10 mg.kg-1 in the condition of 2 h brain ischemia ＋ 24 h reperfusion exhibited a remarkable reduction in the extent of behavior disorder（P〈0. 05, P〈0. 01）, cerebral edema（P〈0. 05, P〈0. 01） and the range of cerebral infarction（P〈0. 05）, meanwhile Sal 40 mg. kg-1 group. Sal 20 rag. kg-1 group and Sal 10 mg. kg-1 group had a obviously increase of SOD, GSH and ATP（P〈0. 05, P〈0. 01 ）and a obviously decrease of MDA and LA in ischemiareperfusion brain compared with focal brain isehemia-reperfusion injury group in aged rats（P〈0. 05, P〈0. 01）. CONCLUSION The present study indicates that salidroside possesses a protective action in aged rats with focal brain ischernia-reperfusion injury by reducing lipid peroxide, scavenging free radicals and improving the energy metabolism.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2011年第9期738-741,共4页
Chinese Journal of Hospital Pharmacy
关键词
老龄大鼠
脑缺血再灌注损伤
能量代谢
氧自由基
红景天苷
aged rats
cerebral ischemia-reperfusion injury
energy metabolism
oxidant free radical
Salidroside
