摘要
目的:建立测定复方酪萨维口腔速崩片中酪萨维和红景天苷含量的方法。方法:采用反相高效液相色谱法。色谱柱为Agilent TC-C18(250mm×4.6mm,5μm),流动相分别为0.2%磷酸溶液-乙腈=86∶14(酪萨维)、甲醇-水=15∶85(红景天苷),柱温为35℃,流速为1.0mL·min-1,检测波长分别为254nm(酪萨维)、275nm(红景天苷)。结果:酪萨维和红景天苷进样量的线性范围分别为0.08～1.60μg(r=0.9998)、0.80～8.00μg(r=0.9993);平均加样回收率分别为100.22%(RSD=1.58%,n=6)、99.37%(RSD=1.35%,n=6)。结论:本方法操作简便、结果准确、专属性强、重复性好,可用于复方酪萨维口腔速崩片的质量控制。
OBJECTIVE：To establish the method for the content determination of rosavin and salidroside in Compound rosavin rapidly disintegrating oral tablets. METHODS：RP-HPLC method was adopted. The determination was performed on Agilent TC-C18（250 mm×4.6 mm,5 μm） column with mobile phases consisted of 0.2% phosphoric acid-acetonitrile（86 ∶ 14） for rosavin and methanol-water（15 ∶ 85） for salidroside at flow rate of 1.0 mL·min-1. The detection wavelength was set at 254 nm and 275 nm,and the column temperature was 35 ℃. RESULTS：The linear ranges of rosavin and salidroside were 0.08～1.60 μg（r=0.999 8） and 0.80～8.00 μg（r=0.999 3） .The average recoveries were 100.22%（RSD=1.58%,n=6） and 99.37%（RSD=1.35%,n=6） ,respectively. CONCLUSION：The method is simple,accurate specific and reproducible,and it is applicable for the quality control of Compound rosavin rapidly disintegrating oral tablets.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第19期1780-1782,共3页
China Pharmacy
基金
国家重大新药创制基金专项课题(2009ZXJ09004-082)
