摘要
目的：研究尿激酶（ Ｕ Ｋ） 或蚓激酶（ Ｅ Ｋ） 与氟罗沙星（ Ｆ Ｌ Ｒ Ｘ） 联合应用对细菌生物被膜的影响。方法：最低抑菌浓度测定采用标准微量稀释法；以银染法快速鉴定细菌生物被膜（ Ｂ Ｂ Ｆ） ；扫描电镜（ Ｓ Ｅ Ｍ） 观察 Ｂ Ｂ Ｆ 形态；用甲基四氮唑蓝（ Ｍ Ｔ Ｔ） 比色法测定 Ｂ Ｂ Ｆ 中活菌数。结果：经 Ｆ Ｌ Ｒ Ｘ 处理 Ｂ Ｂ Ｆ 的 Ｓ Ｅ Ｍ 图像示细菌形态仍保持短杆状，但细菌数量及菌体间纤维样黏液物质显著减少；经 Ｕ Ｋ 或 Ｅ Ｋ 与 Ｆ Ｌ Ｒ Ｘ 合用处理后的 Ｂ Ｂ Ｆ 图像显示细菌破碎、形态不一，菌数和菌体间粘液丝均比单用 Ｆ Ｌ Ｒ Ｘ 时明显减少。对活菌数影响与电镜图像结果一致。结论： Ｕ Ｋ 或 Ｅ Ｋ 与 Ｆ Ｌ Ｒ Ｘ 合用对铜绿假单胞菌 Ｂ Ｂ Ｆ 的抑制具有明显的协同效应。
AIM: To investigate the effects of fleroxacin combined with urokinase or earthworm kinase on the bacterial biofilm (BBF) of Ps.aeruginosa defined. METHODS: MIC(minimal inhibitory concentration) was determined by use of microdilution method, bacterial biofilm was defined by a rapid stainning procedure of AgNO 3, the morphology of biofilm was observed by scanning electron microscopy, viable bacteria were counted by MTT method. RESULTS: The photography of SEM(scanning electron microscopy) showed that the morphology of biofilm bacteria treated with FLRX(fleroxacin) combined with UK(urokinase) or EK(earthworm kinase) was markly changed and the counts of viable bacteria in the BBF(bacterial biofilm) treated with FLRX plus UK or EK were significantly decreased than with FLRX alone. CONCLUSION: The effects on the BBF treated with FLRX plus UK or EK were synergistic.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第9期662-665,共4页
Acta Pharmaceutica Sinica
基金
国家自然科学基金
