摘要
目的：研究川芎中川芎嗪和阿魏酸的毛细管电泳分离分析的可行性。方法：通过用毛细管区带电泳分离、紫外检测模式研究内标、电泳缓冲液、进样方式、分离电压等对样品中川芎嗪和阿魏酸分离与定量的影响，优化实验条件。结果：以对硝基苯甲酸为内标，未涂层熔融石英毛细管（３９５ ｃ ｍ ×５０ μｍ Ｉ Ｄ，有效分离长度３４８ ｃｍ ） 为分离通道，３０ ｍ ｍｏｌ· Ｌ－ １ 硼砂缓冲液（ｐ Ｈ９４３） 为电泳介质，３４ ｋ Ｐａ·ｓ 压力进样，１７ ｋ Ｖ 分离电压，２９５ ｎｍ 检测，川芎嗪和阿魏酸分别在７ ～４２３ μｇ·ｍ Ｌ－ １ 和４ ～９００ μｇ·ｍ Ｌ－ １ 范围内可进行定量检测，回收率分别为１００９ ％ ±１９ ％ 和９９８ ％ ±１０ ％ 。结论：毛细管电泳法可用于川芎中川芎嗪和阿魏酸的同时分离分析。
AIM: To investigate the feasibility of capillary zone electrophoresis (CZE) in the separation and determination of ligustrazin and ferulic acid in Chuanxiong. METHODS: Based on the effects of internal standard ( p nitrobenzoic acid), electrolyte, sampling method and running voltage, etc. on the quantitative assay of ligustrazin and ferulic acid in samples, the optimal conditions by CZE, UV detection were chosen. RESULTS: The experimental conditions are stated as follows: separation column: uncoated fused silica capillary (39 5 cm×50 μm ID, 34 8 cm to UV detector); electrolyte: 30 mmol·L -1 borax, pH 9 43; detection wavelength: 295 nm; sampling: 34 kPa·s; running voltage: 17 kV; temperature: 20℃. The linear ranges of determination for ligustrazin and ferulic acid are 7～423 μg·mL -1 (γ=0 9995, RSD=0 61%～0 89%) and 4～900 μg·mL -1 (γ=0 9992, RSD=0 22%～1 15%) respectively, and the recoveries of ligustrazin and ferulic acid are 100 9%±1 9% (RSD=1 86%, n =6) and 99 8%±1 0% (RSD=1 03%, n =6), respectively. CONCLUSION: CZE is a good method for the simultaneous separation and determination of ligustrazin and ferulic acid in Chuanxiong.
出处
《药学学报》
CAS
CSCD
北大核心
1999年第9期699-701,共3页
Acta Pharmaceutica Sinica
基金
湖北大学95’湖北省生物化学与分子生物学省级重点学科基金
湖北省教委基金
关键词
毛细管电泳
川芎嗪
阿魏酸
川芎
中药
capillary electrophoresis
ligustrazin
ferulic acid
Ligusticum chuanxiong
