摘要
探讨了流动相条件及流速对体积排阻色谱分离抗EGF单克隆抗体的影响.结果表明:选择100mmol/L磷酸钠-150mmol/L氯化钠(pH=7.0)为流动相,设定流速为0.6mL/min时,抗EGF单克隆抗体在体积排阻色谱柱上的分离能获得最佳柱效.
The effect of mobile phase and flow on separation of anti- EGF monoclonal antibody in size exclusion chromatography has been studied. The result showed that anti - EGF monoclonal antibody separated by size exclusion chromatography could have the best column performance in the condition, when selecting 100mM phosphate buffer/150mM sodium chloride (pHT. 0) as mobile phase and setting flow rate 0.6 mL/min.
出处
《周口师范学院学报》
CAS
2011年第2期65-67,共3页
Journal of Zhoukou Normal University
基金
河南省教育厅2011年度自然科学研究项目(2011B150041)
关键词
体积排阻色谱法
抗EGF单克隆抗体
柱效
理论塔板数
size exclusion chromatography
anti -EGF monoclonal antibody
column performance
theoretical plate num-ber