摘要
目的以半夏叶柄为外植体,研究不同植物生长调节剂对半夏愈伤组织形态的影响。方法分别采用单一激素和多个激素正交试验,考察不同植物生长调节剂对半夏愈伤的诱导效果。结果单一激素不同水平的愈伤组织诱导效果因激素种类和浓度的不同而有所差异;多种激素不同水平对半夏愈伤诱导的效果与诱导率和启动时间有关,同时还应考虑愈伤的质地、形态等;植物生长调节剂对半夏愈伤组织的诱导能力从大到小依次为6-苄基腺嘌呤、α-萘乙酸、2,4-二氯苯氧乙酸、蔗糖。结论通过考察不同植物生长调节剂对半夏愈伤组织的诱导效应,确定了适宜做半夏悬浮培养材料的培养基和一次性成苗培养基。
Objective To study the influence of plant growth regulators on callus induction of Pinellia ternata, with the petioles as explants for tissue culture. Methods Single factor experiments and orthogonal experiments were used to study callus induction effects. Results. The induction effect of single plant growth regulators on Pirtellia ternata callus were different depends on hind and concentration. The effect of multiple plant growth regulators was related with induction rate and starting time, as well as the texture and morphology of callus. The effective plant growth regulators were as follows： 6-BA, NAA, 2,4-D sucrose. Conclusion By examining the induction effect of different plant growth regulators on callus of Pinellia ternata, suitable mediums for suspension culture and seedling were determined.
出处
《中国中医药信息杂志》
CAS
CSCD
2011年第5期57-59,共3页
Chinese Journal of Information on Traditional Chinese Medicine
基金
国家自然科学基金(30472151
30672618)
关键词
组织培养
半夏
植物生长调节剂
愈伤组织
tissue culture
Pinellia temate： plant growth regulator： callus
