摘要
采用石蜡永久制片和光学显微摄像的方法,对盘龙参根的显微结构及其内生真菌的分布进行了研究。结果表明：盘龙参的根由表皮、皮层和中柱组成,表皮上有根毛,皮层所占根径的比例达70%以上,其内草酸钙针晶散在或成束于皮层细胞中。中柱由18~22个木质部与韧皮部相间排列组成。盘龙参根的皮层细胞和中柱中有内生真菌的分布;内生真菌由表皮、外皮层侵入到皮层薄壁细胞和中柱的薄壁细胞中,在皮层和中柱中形成菌丝和菌丝结,并扩展成一定的侵染区域,同时在部分皮层细胞中菌丝结已被消化吸收。
Microstructure and distribution of endophytic fungi of the root of Spiranthes sinensis were studied through permanent paraffin-cut section and optical microscoipe photography.The results indicated that the rhizome of S.sinensis consisted of epidermis,cortex and stele.The epidermis had many root hairs,the cortex occupied more than 70% of the diameter of root,in which the cylinder were sometimes observed.Stele were scattered by eighteen to twenty-two xylem and phloem.The hyphae of endophytic fungi were colonized in the cortex and stele.They infected the cortex parenchyma through epidermis and exodermis,and then formed pelotons,and expanded their occupying area of cortex.
出处
《广西植物》
CAS
CSCD
北大核心
2011年第2期194-197,共4页
Guihaia
基金
江西省教育厅科技计划项目(GJJ08410
GJJ09606)~~
关键词
兰科
盘龙参
石蜡切片
光学显微摄像
内生真菌
Orchidaceae
Spiranthes sinensis
permanent paraffin-cut section
optical microscope photography
endophytic fungus
