摘要
以多种胁迫处理的野生型水稻中花11及其携带谷胱甘肽转移酶(GST)和过氧化氢酶(CAT)的功能获得突变体为材料,分析地上部H2O2含量、谷胱甘肽还原酶(GR)活性和同工酶谱的变化.结果显示,胁迫条件下野生型水稻(W)中H2O2的含量明显高于其突变体(M).非胁迫条件下W和M的GR同工酶都有3条带,且W和M之间无显著差异.单一镉、PEG-6000和外源H2O2处理诱导W均产生1条额外谱带,用H2O2和抗坏血酸(AsA)同时处理时,W中则未见该条带.当用镉和CAT抑制剂复合处理时,W和M的所有同工酶活性都降低;而同时用镉和H2O2清除剂处理时,W中则无GR同工酶条带.在其他处理(盐、高温、Zn、盐+高温、镉+高温、PEG+高温和Cd+Zn)条件下,W和M的GR活性和/或同工酶谱的变化也有明显差别.在所有处理中,W和M的GR总酶活性的变化与其同工酶的活性变化基本一致.在胁迫条件下W和M积累H2O2的水平不同,其GR活性和/或同工酶谱的变化也存在明显差异.研究表明,不同环境胁迫诱导水稻GR活性和同工酶谱的变化可能与H2O2水平有关.
The changes of H2O2 content,GR activity and isoenzyme pattern were studied by using wild type（Oryza sativa L.cv ‘Zhonghua 11＇,W） and the gain-of-function mutant（M） of rice（co-expressing glutathione-S-transferase（GST,EC.2.5.1.18） and catalase1（CAT1,EC.1.11.1.6）） treated with multiple abiotic stress.The results showed that the content of H2O2 in W is significantly higher than that in M under all stresses.Three isoenzymes of GR were observed both in W and M under non-stressed conditions,and there was no significant difference between W and M.An extra isoenzyme was induced by cadmium（Cd）,PEG-6000 and exogenous H2O2 in W,respectively.However,this band canot be induced in W by combined H2O2 and ascorbate.When subjected to the combined Cd and 3-amino-1,2,4-triazole（a CAT inhibitor）,the activities of GR isoenzymes decreased in both W and M,but no GR isoforms were observed in W following Cd together with N,N′-dimethylthiourea（a scavenger of H2O2） application.Moreover,the GR isoenzyme patterns and/or activities with other applied stresses were also significantly different（salt,zinc,heat,heat＋salt or Cd or PEG,zinc＋Cd） in W and M.The total GR activity consisted with the increased/decreased expression of GR isoenzymes in W and M under all treatments.These results indicated that the variations of GR activity and isoenzymes under single and combined abiotic stresses in rice may be related to the level of H2O2.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第3期543-551,共9页
Acta Botanica Boreali-Occidentalia Sinica
基金
国家自然科学基金资助项目(30671126)
