摘要
针对市场上存在的高脲乳(掺假和病理性)问题,建立区别于正常生理浓度范围的快速检测方法。检测方法依据尿素被酶解导致的pH值升高幅度与其浓度呈正相关这一机理,对牛乳起始pH值进行优化调整为6.8时,选择溴百里酚蓝作为指示剂,可以使乳脲含量达40mg/100mL生理上限卡切值以上时变色,从而区别检出高脲问题乳。研究表明,依此组装的高脲乳检测试剂盒,可用于生产实践。
The objective of this study is to establish a rapid detection method for milk with urea content exceeding physiological range.Based on the fact that pH increase could result from urea enzymolysis positively correlated with urea concentration.Bromothymol blue was chosen as the acid-base indicator to determine high-urea milk.The indicator can develop color from yellow-brown to green-blue when urea content was higher than 40 mg/100 mL.On the basis of our present study,the developed reagent kit for the determination of high-urea milk is useful for practical application.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第10期222-226,共5页
Food Science
关键词
尿素掺假乳
病理性高脲乳
检测试纸
检测试剂盒
脲酶
乳脲氮
adulterated milk
pathological high-urea milk
test paper
milk-urea reagent kit
urease
milk urea nitrogen
