摘要
比较了12种大孔吸附树脂对板栗壳中原花青素的吸附与解吸性能,在静态吸附研究基础上,筛选出效果较好的树脂进行动态试验,并对所得组分原花青素含量及其相对分子量进行分析。结果表明,AB-8大孔吸附树脂分离纯化CSPCs效果最佳,上样质量浓度为1.864 mg/mL,流速为2 mL/min;当用体积分数为50%乙醇以1 mL/min的流速洗脱4个柱体积时,CSPCs的累积回收率可达94.2%,含量为89.8%。经质谱分析,分子量范围为289.4-1 154.9,聚合度在4以内。
With the aim to separate and purify procyanidins from chestnut-shells（CSPCs） by macroporous resin,twelve macroporous resins were tested for their static adsorption/desorption performance towards CSPCs.Based on the static adsorption experiments,the suitable macroporous resin was screened to carry out dynamic adsorption experiment,the content and the relative molecular weight of CSPCs were analyzed.The results showed that AB-8 was the optimal macroporous resin for separating and purifying CSPCs,the optimum separating condition was as follows： the mass concentration and the sampling rate were 1.864 mg/mL and 2 mL/min,respectively.The total recovery of CSPCs reached 94.2% eluted with 50% ethanol of 4 BV at a flow velocity of 1 mL/min,and the content of the total CSPCs in the obtained fine product was 89.8%.Analysis of CSPCs by ESI-MS demonstrated the molecular weight ranged from 289.4 to 1 154.9（M＋H） within 4 of average polymeric degree.
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2011年第5期156-159,155,共5页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家自然科学基金资助项目(309700309)
国家博士后基金资助项目(20100471379)
江苏省高校自然基金资助项目(05KJB550011)
江苏大学人才基金资助项目(05JDG013
128300289)
江苏高校优势学科建设工程资助项目
关键词
原花青素
板栗壳
大孔吸附树脂
分离纯化
Procyanidins
Chestnut-shells
Macroporous resin
Separation and purification
