摘要
目的:观察宁心痛颗粒对动脉粥样(AS)易损斑块的稳定作用,探讨宁心痛颗粒稳定易损斑块的机制。方法:采用球囊损伤腹主动脉加高脂饲料喂养结合转染p53基因、中国斑点蝰蛇毒和组胺触发建立兔AS易损斑块模型,分别予宁心痛颗粒、血脂康胶囊干预,以普通病理及免疫组化观察斑块形态及NF-κB、MCP-1、MMP1的局部表达;采用Western blot技术检测NF-κB、MCP-1、MMP1水平,进行对比分析。结果:宁心痛颗粒与血脂康胶囊均能够增加纤维帽厚度及纤维帽厚度/IMT比值,降低IMT(P<0.05),两药物作用相当。免疫组化染色结果显示易损斑块模型组AS斑块处均有NF-κB、MCP-1、MMP1的局部表达,表达强度较空白组明显增强(P<0.05),宁心痛颗粒高剂量与血脂康胶囊均能够降低NF-κB、MCP-1、MMP1的表达水平(P<0.05),宁心痛颗粒高剂量与血脂康胶囊作用相当。Western blot检测模型组斑块局部NF-κB、MCP-1、MMP1蛋白水平明显增高(P<0.05),宁心痛颗粒与血脂康胶囊均能够降低NF-κB、MCP-1、MMP1蛋白水平,血脂康胶囊组NF-κB、MMP1水平略低于宁心痛颗粒高剂量组,宁心痛颗粒高剂量组NF-κB、MMP1水平低于宁心痛颗粒常规剂量组(P<0.05)。结论:宁心痛颗粒能够通过抑制炎症反应,从而达到稳定AS易损斑块的目的。
Objective：To investigate the effect of Ningxintong Granule（NXTG）on stabilizing vulnerable plaques and to explore the mechanisms of drug treatment with NXTG in stabilizing vulnerable plaques.Methods：42 healthy male new Zealand white rabbits were randomized into the group A and the group B,rabbits in group B were divided into four subgroups randomly（B1,B2,B3,B4）.Rabbits in group A were fed standard chow.Rabbits in group B were fed high cholesterol diet for 2 weeks and then underwent ballon-induced abdominal aortic wall injure.At the 3rd week,group B1 was NXTG low dose treatment group,B2 was NXTG high dose treatment group,group B3 was administered Xuezhikang,group B4 was baseline（n=9）.Rabbits in group B were still fed the cholesterol-rich diet.At the end of the 8th week,recombinant adenovirus carrying p53 genes were injected through a catheter into the aortic segments rich in plaques in group B.Two weeks later,the remaining rabbits in group B all underwent pharmacological triggering with injection of Chinese Russell＇s viper venom（CRVV） and histamine.The abdominal aorta were processed and examined by HE stain.The levels of NF-Κb,MCP-1 and MMP1 were determined by means of immunohistochemistry,the protein expressions of NF-κB,MCP-1 and MMP1 in the lesions were examined by means of Western-blot.Results：Compared with Group B4,the thickness of cap in medication groups（group B1,B2and B3） was significantly thicker and the intima-media thickness was significantly decreased（P〈0.05）.The ratio of thickness of the plaque cap and intima-media was significantly different between Group B4 and treatment groups（P〈0.05）.Immunohistochemistry showed that expressions of NF-κB,MCP-1 and MMP-1 were found in the atherosclertic plaques.Compared with group B4,the expressions of NF-κB,MCP-1 and MMP-1 showed by immunohistochemistry in the group B2 and B3 were much lower（P〈0.05）,but there was no significant difference between group B2 and group B3.The protein expressions of NFκB,MCP-1 and MMP1were found in the atherosclerosis lesions.Compared with group B4,the protein expressions of NFκB,MCP-1 and MMP1 in group B2 and B3 were much lower in the atherosclerosis lesions（P〈0.05）.Compared with group B1,the expressions of NFκB,MCP-1 and MMP1 in the group B2 were lower（P〈0.05）.Conclusion：NXTG has effects of anti-inflammation leading to stabilization of vulnerable plaques.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第5期1042-1046,共5页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
江苏省中医药局资助项目(No.HZ07033)
南京市科技局科技发展计划项目(No.200801081)
南京市卫生局科研基金资助项目(No.YKK07085)~~
关键词
动脉粥样硬化
易损斑块
宁心痛颗粒
机制研究
Atherosderosis
Vulnerable plaques
Ningxintong Granule
Mechanism study
