摘要
目的:探讨三七总皂苷(Panax notoginseng saponins,PNS)干预链脲佐菌素(Streptozotozin,STZ)诱导的大鼠糖尿病肾病(Diabetic nephropathy,DN)氧化应激及足细胞凋亡机制。方法:体内实验:44只SD雄性大鼠,单侧肾切除1周后一次性腹腔注射55mg/kg STZ造模,造模后,按体质量随机分为正常组,模型组,厄贝沙坦组(IRB组),三七总皂苷组(PNS组),每2周测体质量、24h时尿蛋白定量,12周后处死,记录肾质量体质量比;检测血清TG、BUN、Scr、NO、MDA和SOD浓度;进行肾组织HE、mallory、masson、六胺银染色,观察肾组织病理变化并评分;免疫组化检测肾组织TGF-β1、Caspase-3蛋白表达;原位杂交检测肾组织TGF-β1 mRNA表达。体外实验:用RT-PCR检测PNS对高糖刺激下体外培养足细胞Caspase-3 mRNA表达的影响。结果:与模型组比较,PNS组第8、10、12周体质量显著增加(P<0.05),肾组织病理评分、肾重体重比、血清TG、BUN、Scr等指标明显改善(P<0.05),血清SOD活性增强(P<0.05),同时MDA含量减少(P<0.05),肾组织TGF-β1 mRNA、Caspase-3蛋白水平下调(P<0.05);体外培养PNS组足细胞Caspase-3 mRNA表达减少。结论:PNS可能通过抗氧化应激抑制足细胞凋亡而对STZ诱导的大鼠DN起到一定的保护作用。
Objective：To observe the antioxidant effects of PNS on DN rat model and high glucose cultured cell.Methods：Unilateral nephrectomy and intraperitoneal injection of STZ（55mg/kg） was used to build diabetic rats that were divided into normal group,model group,IRB group,PNS group.Rat body weight,24-hour urine protein was test at 12 weeks,measured kidney weight body weight ratio,levels of serum TG,BUN,Scr,NO,MDA and SOD concentration;kidney HE,mallory,masson,methenamine silver staining;immunohistochemistry detection of renal TGF-β1 and Caspase-3 protein;RT-PCR detection of renal TGF-β1 and podocyte Caspase-3 mRNA.Results：Compared with model group,the kidney weight/body weight ratio of PNS group were significantly decreased（P〈0.05）;the urine protein of PNS group was significantly decreased（P〈0.05） at the end of the 4,8,12week（P〈0.05）.PNS also decrease the biochemical parameters such as BUN,Scr and TG.PNS groups SOD,NO were significantly increased（P〈0.05）.TGF-β1 mRNA levels was significantly lower（P〈0.05） compared with model group,Caspase-3 gene level were significantly reduced（P〈0.05）.Conclusion：Regulating renal TGF-β1,Caspase-3 gene expression may be one of the mechanisms of PNS therapeutic effect of DN.
出处
《中华中医药杂志》
CAS
CSCD
北大核心
2011年第5期1062-1067,共6页
China Journal of Traditional Chinese Medicine and Pharmacy
基金
国家自然基金项目(No30973831)
北京市中医管理局"51510"资助项目(No JJ2007-006)~~
