摘要
尿素可以持续地从健康人的唾液和龈沟液中分泌出来,被口腔细菌尿素酶快速水解,产生的氨能够升高口腔环境的pH值,引起菌斑生物膜的改变,从而减少龋齿的发生。由于尿素水解作用对口腔环境中微生物致病机制的重要影响,所以对尿素酶及其活性的检测对预防和治疗龋齿可能具有很大的帮助。现分别从定性检测、半定量检测、定量检测3个不同层面,将近年来报道的口腔细菌尿素酶的检测方法作一综述。
Urea can be continuously secreted out of the healthy individuals＇ saliva and gingival crevicular fluid,and it can be rapidly hydrolysed by ureases of oral bacteria.Ammonia generated by ureolysis can increase the pH value of oral environment,and this causes changes in dental plaque biofilms,thus reduces the initiation and progression of dental caries.As the significant impaction of ureolysis on the microbial pathogenesis in the oral cavity,the detection of urease activity might have a great help with the prevention and treatment of dental caries.This review will describe the detection methods of oral bacterial ureases reported in recent years from three different levels： Qualitative test,semi-quantitative test,and quantitative test respectively.
出处
《国际口腔医学杂志》
CAS
2011年第3期358-360,共3页
International Journal of Stomatology
基金
国家自然科学基金资助项目(30572038)
上海市自然科学基金资助项目(08ZR1416800)
上海市重点学科建设基金资助项目(S30206)
上海市科学技术委员会基金资助项目(09DZ22-72100)
关键词
口腔细菌
尿素酶
检测
龋齿
oral bacteria
urease
detection
dental caries
