摘要
目的:探讨P57和Ki-67蛋白在完全性和部分性葡萄胎鉴别诊断中的作用。方法:分别收集正常胎盘绒毛、部分性葡萄胎和完全性葡萄胎石蜡标本各12例,应用免疫组织化学方法检测P57和Ki-67蛋白在这些病变中的分布及表达水平。结果:P57蛋白在正常绒毛及部分性葡萄胎组织中主要分布于绒毛的细胞滋养叶细胞及间质细胞,两组间阳性率未见明显差异;而在完全性葡萄胎中细胞滋养叶细胞及间质细胞P57表达缺失。Ki-67蛋白主要表达于绒毛的细胞滋养叶细胞。正常绒毛组、部分性葡萄胎组和完全性葡萄胎组Ki-67阳性细胞百分率比较差别具有统计学意义(P<0.05)。结论:P57结合Ki-67免疫组织化学检测可有效鉴别完全性和部分性葡萄胎。
Objective To investigate the role of P57 and Ki-67 protein in the differential diagnosis of partial or complete hydatidiform mole.Methods Tissues of normal placental villi,partial hydatidiform mole,and complete hydatidiform mole（12 for each） were collected.Distribution and expression of P57 and Ki-67 proteins were detected by immunohistochemistrical assay.Results P57 expression was located in the nuclei of villous cytotrophoblasts and stromal cells.There was no significant difference in distribution of P57 positive cells between normal villi and partial hydatidiform mole.However,none of the complete hydatidiform mole exhibited P57-positive cells.The Ki-67 expression was observed in the nuclear of cytotrophoblastic cells within placental tissues.There were significant differences in Ki-67-positive rate among normal placenta villi,partial hydatidiform mole,and complete hydatidiform mole（P〈0.05）.The Ki-67-positive rate in complete hydatidiform mole was higher than that in partial hydatidiform mole（P〈0.01）.Conclusion The combined use of P57 and Ki-67 protein examination might be helpful to distinguish the complete hydatidiform mole from the partial hydatidiform mole.
出处
《国际病理科学与临床杂志》
CAS
2011年第2期117-120,共4页
Journal of International Pathology and Clinical Medicine
