摘要
目的:建立小鼠慢性非细菌性前列腺炎模型,探讨浙贝母治疗慢性前列腺炎临床疗效。方法:参考Robinette的方法选取25-35g昆明种小鼠,于第1、14天分别用小鼠前列腺匀浆+免疫佐剂(饱和氢氧化铝)溶液0.5 mg/只皮下注射,观察实验小鼠前列腺组织的大体病理表现。给药组每日浙贝母灌胃,每只0.1mL/10g给药,7天后观察浙贝母对大鼠病理学与血清NO的影响。结果:建模14天后,模型组小鼠前列腺出现不同程度的干湿重增加、组织粘连等大体病理学改变。与模型组相比,给药组小鼠则具有较轻的炎症表现,差异均呈显著性(P<0.05)。NO含量亦呈相同趋势变化。结论:运用小鼠前列腺匀浆+免疫佐剂饱和氢氧化铝溶液方法建立了慢性非细菌性前列腺炎模型,为以后进一步研究前慢性非细菌性前列腺炎的发病机理以及临床用药提供了另一种方法。浙贝母则有减轻慢性非细菌性前列腺炎动物模型炎症表现的作用。
Objective：To develop a mice model of nonbacterial chronic prostatitls. To explore the treatment of chronic prostatitis Pui the mechanism to evaluate its clinical efficacy prostatitis. Methods ： Aged Sprague - Dawley mices were castrated and injected Aluminum Hydroxide immune file. （0.5 mg/day, s. C. ） for 30days. Prostates were harvested after 30 days injection. Prostatic tissue was examined histologically for degree of inflammation. Pui to observe the impact of pathological model. Results ： Different degree of inflammation and lymphocytic infiltration was found in prostate after injection. Endovascular secretions significantly increased. Interstitial infiltration of inflammatory cells was decreased fibrosis alsodecreased noticeably. Compared with model group, there was a significant difference （P 〈0. 05）. Most animals laminin （LN） and fibrenectin （FN） was weakly positive, a few animals positive linear gland in the basement membrane, intersti- tial and perivascular Flake point spread in Leydig, Compared with model group, there was a significant difference （P 〈 O. 05 ）. Conclusion：Injection of estradiol benzoate can induce prostatic inflammation similar to clinical nonbacterial chronic prostafitis and it will be a good way to found an animal prostatitis model. Big Shell is reducing pathological model inflammatory response, reducing the degree of fibrosis.
出处
《中华中医药学刊》
CAS
2011年第5期1023-1025,共3页
Chinese Archives of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(30973003)
