摘要
[目的]了解BIOLOG自动微生物鉴定系统对水产动物病原菌鉴定结果的可靠性,为水产动物细菌性疾病的诊断提供参考。[方法]选用BIOLOG自动微生物鉴定系统对9株分离自发病水产动物的病原菌进行鉴定,同时采用16S rDNA序列分析对其中的4株进行鉴定,比较2种鉴定结果的符合率。[结果]采用自动微生物鉴定系统对9株病原菌进行鉴定均取得良好结果,采用16S rDNA序列分析对4株病原菌进行鉴定的结果与应用自动微生物鉴定系统所得结果完全一致,二者符合率为100%。[结论]应用BIOLOG自动微生物鉴定系统对水产动物病原菌进行鉴定具有操作简便、快速、准确等特点,是实验室鉴定水产动物病原菌的一种可行方法。
[Objective] The aim was to know the reliability of BIOLOG automatic microbiological assay system in the detection of aquatic animals＇ pathogen,providing references for the diagnosis of aquatic animals＇ bacteriosis.[Method] 9 strains pathogen isolated from diseased aquatic animals were identified by BIOLOG automatic microbiological assay system,while 4 strains of them were identified by 16S rDNA sequencing and phylogenetic analysis,then two identification results of them were compared.[Result] The results of the 9 strains identified by BIOLOG automatic microbiological assay system were very satisfactory,and the results of the 4 strains which were identified simultaneously by above two methods were coincident completely.[Conclusion] It was rapid,accurate and convenient to detect pathogen in aquatic animals with the application of BIOLOG automatic microbiological assay system.
出处
《安徽农业科学》
CAS
北大核心
2011年第13期7846-7848,共3页
Journal of Anhui Agricultural Sciences
基金
广西科技攻关项目(桂科攻0992015-1)
