摘要
[目的]对宁夏六盘山区黄牛杂交改良群体生长激素受体(GHR)基因的多态性进行分析,为宁夏黄牛杂交改良选育提供理论参考。[方法]采用聚合酶链式反应-限制性片段长度多态性方法(PCR-RFLP)对该群体70个个体的GHR基因多态性进行检测。[结果]扩增出了338 bp的目的片段,PCR产物被限制性内切酶AluI消化后表现出多态性,其中AA基因型频率为75.71%;BB基因型频率为24.29%。[结论]共检测到AA和BB 2种基因型,六盘山区黄牛杂交改良群体GHR基因具有多态性。
[Objective] The aim was to analyze the polymorphism of the growth hormone receptor（GHR） in improved hybrid yellow cattle from Liupan Mountain area in Ningxia Autonomous Region,to provide technological basis for hybrid improvement.[Methods] Polymerase chain reaction-restriction fragment length polymorphisms（PCR-RFLP） technology was carried out to examine polymorphisms of GHR gene of 70 individuals.[Result] The target fragment of 338 bp was amplified.After digested by restriction enzyme Alu I,the PCR product showed polymorphisms.The frequencies of the two genotypes（AA,BB） were 75.71%（53 individuals） and 24.29%（17 individuals）,respectively.[Conclusion] Two genotypes of GHR gene were detected in improved hybrid yellow cattle from Liupan Mountain area in Ningxia.
出处
《安徽农业科学》
CAS
北大核心
2011年第13期7854-7856,7889,共4页
Journal of Anhui Agricultural Sciences
基金
宁夏科技攻关计划项目(5183003)
科技人员服务企业行动项目(2009GJG30036)
