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应用基因芯片技术筛选乳腺浸润性导管癌差异表达基因 被引量：1

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摘要 目的:应用基因芯片技术研究乳腺浸润性导管癌(invasive ductal carcinoma,IDC)基因表达的改变。方法:收集手术切除3例IDC癌组织及癌旁对应正常组织标本,提取总RNA,逆转录合成cDNA,用荧光素Cy3通过体外转录分别将6份标本的cDNA标记成cRNA探针,并与6张基因芯片Illumina Human WG-6 expression beadchip杂交,利用荧光扫描仪扫描荧光强度并分析基因表达谱的差异。结果:按差异显著性标准,在3对标本中共同差异表达的基因有6 756个,表达上调3 927个,表达下调2 829个。结论:IDC的发生、发展存在多基因表达调控的改变,基因芯片技术可同时定量研究大量基因表达水平,为认识肿瘤发病机制和个体化治疗提供生物学依据。
作者 冼书林 陆云飞 陈忠彪 刘冠群 张海添
机构地区 广西医科大学第一附属医院胃肠腺体外科
出处 《广西医科大学学报》 CAS 2011年第1期106-109,共4页 Journal of Guangxi Medical University
基金 广西医疗卫生重点科研课题(No.卫重200635)
关键词 乳腺肿瘤 基因芯片 基因表达
分类号 R737.9 [医药卫生—肿瘤]
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参考文献9

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共引文献11

同被引文献15

  • 1孙建国,廖荣霞,周度金,陈正堂.基于基因芯片的乳腺癌干细胞miRNAs检测分析[J].重庆医学,2007,36(13):1280-1282. 被引量：13
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  • 4Casimiro MC,Crosariol M,Loro E. ChIP sequencing of cyclin D1 reveals a transcriptional role in chromosomal instability in mice[J].Journal of Clinical Investigation,2012,(03):833-843.
  • 5Budczies J,Weichert W,Noske A. C,enome-wide gene expression profiling of formalin-fixed paraffin-embedded breast cancer core biopsies using microarrays[J].Journal of Histochemistry and Cytochemistry,2011,(02):146-157.
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  • 9Smeets A,Daemen A,Vanden Bempt I. Prediction of lymph node involvement in breast cancer from primary tumor tissue using gene expression profiling and miRNAs[J].Breast Cancer Research and Treatment,2011,(03):767-776.
  • 10Morrison C,Mancini S,Cipollone J. Microarray and proteomic analysis of breast cancer cell and osteoblast co-cultures:role of ostcoblast matrix metalloproteinase (MMP)-13 in bone metastasis[J].Journal of Biological Chemistry,2011,(39):34271-34285.

引证文献1

广西医科大学学报
2011年 第1期

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