摘要
目的观察聚乳酸-乙醇酸聚合物[Poly(DL-lactic-co-glycolic acid),PLGA](LA∶GA=75∶25)与大鼠嗅鞘细胞的生物相容性。方法将分离提纯的大鼠嗅鞘细胞接种于PLGA膜上,对照组以相同的细胞接种于多聚赖氨酸包被的圆玻片上。使用倒置显微镜、扫描电镜观察细胞的黏附和生长情况,并采用MTT法及荧光染色后电子图像统计分析检测支架对细胞的毒性。结果嗅鞘细胞接种于PLGA膜上后良好生长,S-100阳性细胞计数、胞体面积和细胞周长与对照组无显著差异(P>0.05)。结论 PLGA聚合物与嗅鞘细胞生物相容性良好,有望用于脊髓损伤修复的组织工程研究。
Objective To explore the biocompatibility of Poly（DL-lactic-co-glycolic acid）（PLGA）（LA∶GA=75∶25） with olfactory ensheathing cells（OECs） in rat.Methods The purified OECs were seeded on the PLGA membrane（PLGA group） and on the columns coated Poly-L-Lysine（control group）.The adhesion and viability of OECs were observed by inverted microscope and scanning electron microscopy.MTT method and the computer image statistical software were used to determinate the survival and proliferation of OECs.Results OECs grew well on PLGA membrane.There were no significant differences in the activity of OECs.The number of S-100 positive cells,the area of the cell bodies and the perimeter of the cell between two groups（P0.05）.Conclusion The PLGA biomaterial has good biocompatibility with rat OECs.It could be an ideal tissue engineered scaffold material in the repair of spinal cord injury.
出处
《组织工程与重建外科杂志》
2011年第2期80-84,共5页
Journal of Tissue Engineering and Reconstructive Surgery
基金
江苏省"医学重点人才"资助项目(RC2007027)
南通市社会发展科技计划项目(S2009014)
关键词
嗅鞘细胞
聚乳酸-乙醇酸聚合物
生物相容性
脊髓损伤
Olfactory ensheathing cells
Poly（DL-lactic-co-glycolic acid）
Biocompatibility
Spinal cord injury
