摘要
The enzyme activities of phenylalanine ammonia-lyase from six years old Ginkgo biloba leaves in the potted orchard were induced by four groups of metal ions respectively. The PAL activities and flavonoids content were measured by the UV-Vis spectrophotometer. Changes of the whole year were also studied. The results showed that four groups of metal ion treatments had significant effects on the PAL activities and flavonoids contents in Ginkgo leaves. The treatments with 0.1% and 0.3% FeSO4, 0.1%, 0.2% and 0.3% ZnSO4, 0.1%, 0.3% and 0.5% MnSO4, 0.1% and 0.4% CuSO4 not only enhanced flavonoids contents, but also extended the optimum harvest dates of ginkgo leaves. The optimum combination was 0.1% FeSO4, 0.2% ZnSO4, 0.3% MnSO4, and 0.4% CuSO4. It could improve flavonoids content up to 1.619 4%.
基金
Acknowledgments： This work was funded by the New Century Talent Support Program （No. NCET-04-0746） and the Region Technology Development Program of Chinese Ministry of Education （No. 02095）, and the Natural Science Foundation of Hubei province （No. 2002AB094）, the Youth Talent Foundation of Hubei province （No. 2003AB014） and the Educational Office Key Research Program of Hubei Province of China （No. Z200627002）.
