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CTGF在兔眼深层巩膜切除术中的表达 被引量:1

Expression of CTGF in rabbits deep sclerectomy surgery
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摘要 目的:探讨结缔组织生长因子(connective tissue growthfactor,CTGF)在兔眼深层巩膜切除术(deep sclerectomy,DS)中的表达。方法:制备人去内皮冻干辐照脐带静脉管(HUV);选取28只大耳白兔,其中24只按自身对照原则,双眼均行深层巩膜切除术,随机选取一眼于术中植入HUV,为植入组;另一眼不植入,为未植入组。分别于术后3,7,14d各处死8只;另4只8眼做正常对照组。应用半定量PCR检测兔眼深层巩膜组织中CTGF mRNA的表达,免疫组织化学检测CTGF蛋白的表达。结果:植入组3d后,CTGF mRNA和蛋白表达开始升高,至7d表达水平达到峰值,14d时表达水平开始下降,明显高于对照组(P<0.01),未植入组中不同时间点表达水平变化与植入组类似。植入组与未植入组比较无统计学意义(P>0.05)。结论:HUV在兔眼DS中没有明显的抗纤维增殖的作用,其瘢痕增殖的过程与正常瘢痕形成的过程是一致的。其维持减压室作用机制可能是机械引流的作用结果;HUV作为一种植入物,并未刺激局部的瘢痕过度形成。 ·AIM:To investigate the expression of connective tissue growth factor(CTGF)in rabbits deep sclerectomy(DS)surgery.·METHODS:Human umbilical vein(HUV)was treated with freeze-drying for 6 hours and ^(60)Co200cGy irradiation for 30 seconds.The samples were stored in RT.28 rabbits were used,24 of which were performed DS surgery in both eyes.Then one eye was randomly selected to plant HUV according to self-control rule,which composed experimental group.The other eye of the 24 rabbits composed control group.8 rabbits were sacrificed 3,7 and 14 days after operation,respectively.The 8 eyes of the other 4 rabbits were used as normal control.Semi-quantitative RT-PCR and immunohistochemistry were used to assess the expression of CTGF mRNA and protein of deep sclera.·RESULTS:The levels of CTGFmRNA and protein expression in the normal control tissue were lower,but at day 3 they increased more quickly than the control and rapidly peaked at day 7,then decreased at day 14(P0.01);there were no significant differences at same time points between experimental group and control group(P0.05).·CONCLUSION:These indicated that DS surgery with huaman freeze-dried allograft umbilical vein implant is safe and effective.It has no evident effect of anti-fiber proliferation.The mechanism for its maintanence of decompression chamber may be the result of mechanical water spreading.·
出处 《国际眼科杂志》 CAS 2011年第5期795-797,共3页 International Eye Science
关键词 结缔组织生长因子 深层巩膜切除术 人脐带静脉管 connective tissue growth factor deep sclerectomy surgery human umbilical vein rabbit
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同被引文献11

  • 1郭长梅,韩泉洪,赵俊宏,惠延年,王静波,贾洪真,马吉献.结缔组织生长因子在体外人视网膜色素上皮细胞损伤模型中的表达和促移行作用[J].中华眼底病杂志,2005,21(5):306-309. 被引量:5
  • 2Zhang H, Li C, Peter CB. Expression of intergins and MMPs during aikaline-burn-induced corneal angiogenesis. Invest Ophthalmol Vis Sci ,2002,43 (4) :955-962.
  • 3Bradham DM, Igarashi A, Potter RL, et al. Connective tissue fac- tor:a cysteine- rich mitogen secreted by human vascular endotheli- al cells is related to the SRC- induced immediate early gene prod- uct CEF-10. Cell Biol, 1991, 114(6) :1285 -1294.
  • 4Kim KH ,Park GT, Lim YB, et al. Expression of connective tissue growth factor, a biomarker in senescence of hunan diploid fibro- blasts ,is up-regulated by a transforming growth factor-bera-medi-a- ted signaling pathway. Biochem Biophys Res Commun,2004,318 (4) :819-825.
  • 5Kuiper E J, Van N ieuw enhoven FA, de Smet MD, et al. The an- gio fibrotic switch of VEGF and CTGF in proliferativc diabetic reti- nopathy. PLos One 2008 ; 3 (7) : 2675.
  • 6Chudgar SM, Deng P, Maddala R, et al. Regulation of connective tissue growth factor expression in the aqueous humor outflow path- way. Molecular Vision, 2006, 12(10) :1117-1126.
  • 7Klenkler B, Sheardown H. Growth factors in the anterior seg- ment: role in tissue maintenance, wound healingand icular pa- thology. Exp Eye Res ,2004,79 (5) :677-688.
  • 8田海霞,王超英.结缔组织生长因子在眼科疾病中的研究进展[J].临床误诊误治,2010,23(2):184-186. 被引量:2
  • 9翁夷平,赵平.低分子肝素对大鼠角膜碱烧伤后血管内皮生长因子和肝细胞生长因子表达的影响[J].中华眼科杂志,2010,46(3):268-270. 被引量:3
  • 10王军花,高桂平.角膜新生血管的发生与治疗[J].中国组织工程研究与临床康复,2010,14(33):6223-6225. 被引量:6

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