摘要
目的 探讨粉防己碱（Ｔｅｔｒａｎｄｒｉｎｅ，Ｔｅｔ） 对胆汁性肝纤维化的保护作用及其保护机理。方法 复制大鼠胆汁性肝纤维化模型，灌胃给予Ｔｅｔ３０ｍｇ／ｋｇ 体重，每天１ 次；在胆道梗阻后１０、２０、３０天，重复检测肝细胞线粒体钙含量，肝组织丙二醛（ＭＤＡ）、超氧化物岐化酶（ＳＯＤ） 含量，血清白蛋白（ＡＬＢ）及透明质酸（ＨＡ）含量变化；并观察肝脏显微及超微结构改变。结果 各Ｔｅｔ 治疗组与同时相的单纯梗阻组比较，血清ＨＡ水平下降，Ｐ＜ ０．０５；ＡＬＢ升高，Ｐ＜ ０ ．０５；肝细胞线粒体钙超载程度明显减轻，Ｐ＜０．０１ ；肝组织ＭＤＡ含量减少，Ｐ＜ ０．０１，ＳＯＤ含量升高，Ｐ＜ ０．０５。光镜及透射电镜下，肝纤维化程度明显减轻。结论 Ｔｅｔ 对胆汁性肝纤维化有明显的保护作用，其保护机理可能与其钙拮抗和抗脂质过氧化损伤作用有关。
Objective To explore the protective effects and the protective mechanism of tetrandrine on hepatic fibrosis due to biliary obstruction. Methods A model of biliary hepatic fibrosis was established in rats by ligating the common bile duct and a section between the ligations.Tetrandrine was administered once a day by gavage at a dose of 30mg/kg. At ten, twenty and thirty days after complete biliary obstruction the calcium content of mitochondria ,the contents of liver malondialdyhyde( MDA) and superoxide dismutase(SOD),the concentration of serum ALB and HA were monitored.Hepatic morphological changes were observed by light and electron microscopies. Results Compared with untreated group with the same obstruction time, serum ALB of every tetrandrine treated group increased (p<0.05), serum HA decreased (p<0.01), and mitochondrial calcium content decreased(p<0.01) all significantly, while liver MDA decreased (p<0.01) and liver SOD increased (p<0.05) significantly. Hepatic fibrosis was markedly reduced. Conclusions Tetrandrine can protect liver effectively against fibrosis due to biliary obstruction. The protective mechanism might be due to its effects of calcium antagonism and anti-lipid peroxidation.
出处
《中华肝胆外科杂志》
CAS
CSCD
1999年第5期317-320,共4页
Chinese Journal of Hepatobiliary Surgery
关键词
胆道梗阻
肝纤维化
粉防己碱
保护作用
Biliary obstruction Hepatic fibrosis Tetrandrine Protection
