摘要
目的探讨燃煤型氟中毒对大鼠海马齿状回星形胶质细胞的影响,为地方性氟中毒引起脑损伤的发病机制提供实验依据。方法取断乳2周的SD大鼠90只(平均体重91.1 g,80～100 g),按体重随机分为正常对照组、低氟组(3.3mg/kg)、高氟组(106 mg/kg),每组30只,雌雄各半。除对照组食用正常饲料外,其他各组均食用不同配方饲料,复制氟中毒大鼠模型。模型建立后,取海马组织用常规石蜡切片,行胶质原纤维酸性蛋白免疫组化染色,计数海马齿状回GFAP免疫组化染色阳性反应细胞,并用细胞形态学计量方法测量各阳性反应产物的平均光密度;通过透射电镜观察海马齿状回区星形胶质细胞的细微结构。结果氟中毒组大鼠的主要变化有GFAP免疫组化染色阳性细胞明显多于对照组(P<0.05),阳性反应产物平均光密度明显高于对照组(P<0.05)。结论实验结果提示燃煤型氟中毒大鼠可导致其海马齿状回星形胶质细胞增多;氟可导致大鼠神经发育障碍。
Objective Probe the effect of astrocyte of morphological and ultrastructure of rat dentate gyrus in hippocampus by fluorosis rats caused by coal burning,for the basis of the pathogenesy for endemic fluorosisthe.Methods 90 SD rats of two week after ablactation were divided into control group,low fluorine group（3.3mg/kg）,high fluorine（106 mg/kg） at random,30 of each groups.Besides the normal control groups were feeded on normal diet,other two groups were feeded on different prescription die for duplicating fluorine poisoning models.Then hippocampal tissue slice were preparated by the way of regular paraffin embedding,HE staining and GFAP Immunohistostaining were performanced respectively,and counting the GFAP positive cells and measuring average opticaldensity of the GFAP-positive reaction of each groups by the methods of norphometry.And the ultrastructure of astrocyes in dentate gyrus were observed by the transmission electron microscope.Results The quantity of GFAP-positive cells of fluoride poisoning group has been much more than the control group（P0.05）,and the average opticaldensity of the GFAP-positive reaction of the fluoride poisoning group has been remarkably higher than the control group（P0.05）,and there has been noticeable distinction between the high dose groups and the low dose groups（P0.05）.And there has been distinction of the ultrastructure characteristic of astrocytes in each groups.Conclusions Experimental result indicates that fluorine poisoning by coal burning might lead to reactive hyperplasia of astrocytes of dentate gyrus in hippocampus,and fluorine might influence the development of rat＇s central nervous system.
出处
《中国地方病防治》
2011年第2期99-102,共4页
Chinese Journal of Control of Endemic Diseases
