摘要
采用索氏提取法对菘蓝根、茎、叶、花和幼果等不同器官的脂溶性成分进行提取,利用气相色谱-质谱联用(GC-MS)技术对其脂溶性成分进行鉴定,并运用面积归一化法确定相对含量,以明确菘蓝的化学物质基础,为综合开发利用菘蓝资源提供依据。结果显示:从菘蓝的根、茎、叶、花和幼果中分别鉴定出24、23、21、18和23种脂溶性成分;其中,十二烷、十三烷、十四烷、2,4-二叔丁基苯酚和棕榈酸为各器官的共有成分;不同器官中脂肪酸含量依次为:花(80.91%)>根(60.56%)>幼果(21.93%)>茎(17.45%)>叶(11.28%)。除花中的饱和脂肪酸含量高于不饱和脂肪酸外,其他器官中不饱和脂肪酸的含量均较高,表明菘蓝不同器官中脂溶性成分的组成及含量存在一定差异。
The fat-soluble components of different organs from Isatis indigotica Fort.were extracted by Soxlet extraction methods,and analyzed and identified by GC-MS.There are 24,23,21,18 and 23 kinds of components were identified from radix,stem,leaf,flower and fruitlet,respectively,of which included 5 kinds of common compounds,such as dodecane,tridecane,tetradecane,2,4-bis（1,1-dimethylethyl）-phenol and hexadecanoic acid.The relative contents of fatty acid from different organs were in the following order：flower（80.91%）radix（60.56%）fruitlet（21.93%）stem（17.45%）leaf（11.28%）.Expect for flower,the unsaturated fatty acids contents were higher than saturated fatty acids contents in other organs.There are obvious differences among composition and relative contents of fat-soluble components in different organs from I.indigotica.Our results will further reveal the chemical constitutes of I.indigotica and provide certain experimental basis on exploitation for the resources.
出处
《西北植物学报》
CAS
CSCD
北大核心
2011年第4期823-828,共6页
Acta Botanica Boreali-Occidentalia Sinica
基金
陕西省科技攻关计划(2009K19-07)
关键词
菘蓝
脂溶性成分
索式提取
GC-MS
Isatis indigotica Fort.
fat-soluble components
Soxlet extraction
GC-MS
