摘要
急性白血病的出凝血异常和组织因子(tissue factor,TF)的高表达有关。循环血流中的TF主要表达于细胞、微粒(microparticle,MP)和异常剪接TF(alternatively spliced human tissue factor,asHTF)中。为探究asHTF在急性髓系白血病(acute myeloblastic leukemia,AML)出凝血异常中的作用,本研究选用了6种常见的AML细胞株NB4、HL-60、Kasumi-1、U937、K562和THP-1,在RNA水平进行了RT-PCR检测。结果发现,在6种细胞株中仅NB4、U937细胞存在全长TF和asHTF的RNA水平基线表达并经测序验证,对上述2种细胞在蛋白质水平进行了流式细胞仪及TF活性、抗原检测发现,asHTF仅有极少量TF抗原表达且基本无TF活性,而MP相关TF(microparticle associated tissue factor,MP-TF)的抗原和活性显著高于asHTF。结论:在NB4、U937细胞中asHTF基本无促凝作用,而MP-TF有凝血活性且在肿瘤细胞释放TF中占主导地位。
The high expression of tissue factor（TF） is related to the coagulation disorder in acute leukemia.TF in blood circulation is mainly expressed in cells,microparticles（MP） and alternatively spliced human tissue factor（asHTF）.To elucidate the role of TF in the coagulation disorder of acute myeloid leukemia（AML）,RT-PCR was performed on 6 common AML cell lines NB4,HL-60,Kasumi-1,U937,K562 and THP-1.The results showed that only NB4 and U937 cells expressed baseline full-length TF and asHTF which were proved by sequencing.The flow cytometric detection,TF activity and TF antigen tests in NB4 and U937 cells revealed that the asHTF was expressed in trace amount and almost had no activity,while the TF antigen and activity in microparticles were significantly higher than that in asHTF.It is concluded that asHTF may play an unimportant role in the coagulation disorder of AML.Microparticle associated tissue factor（MP-TF） is the predominant source of TF activity released from AML cells.
出处
《中国实验血液学杂志》
CAS
CSCD
2011年第2期288-292,共5页
Journal of Experimental Hematology
基金
国家自然科学基金资助项目(编号30801062)
关键词
急性髓系白血病
组织因子
异常剪接组织因子
微粒
acute myeloid leukemia
tissue factor
alternatively spliced human tissue factor
microparticle
