摘要
目的:探讨超氧化物歧化酶(SOD)活性以及一氧化氮(NO)、丙二醛(MDA)和羟自由基(OH·)含量的变化与H3N2猪流感病毒诱导的小鼠肺损伤的关系。方法:选用6-8周龄、SPF级BALB/c小鼠80只,随机分为H3N2病毒感染急性肺损伤组和模拟感染对照组,每组各40只。在感染后的第3、5、7、10和14d,每组处死小鼠6只,做如下处理:其中2只小鼠取肺组织进行HE染色,观察肺组织的病理学变化;剩余4只小鼠处死,收集血液分离血清;然后进行支气管肺泡灌洗,收集支气管肺泡灌洗液(BALF)。测BALF和血清内SOD活性以及NO、MDA和OH·含量。结果:病毒感染小鼠肺脏组织学变化表现为以严重的肺泡间质水肿、炎性细胞渗出、出血为特征的弥漫性肺泡损伤;与模拟感染对照组相比,感染组BALF与血清内的NO、MDA和OH·的含量均明显增加,差异显著;感染组SOD活性与对照组相比显著下降。BALF内SOD、NO、MDA和OH·的变化幅度明显大于血清的变化。结论:感染小鼠血清和BALF内NO、MDA和OH·显著升高,表明在H3N2猪流感病毒诱导的小鼠肺损伤过程中上述自由基可能发挥重要作用。
AIM：To investigate the relationship between acute lung injury induced by H3N2 swine influenza virus and the changes of superoxide dismutase（SOD）,nitric oxide（NO）,malondialdehyde（MDA）and hydroxyl radical （OH·）in mice.METHODS：Eighty BALB/c mice（6 to 8 weeks old）were divided into H3N2-infected group and mock-infected group.The mice in H3N2-infected group were intranasally inoculated with A/swine/Hebei/2/2008 （H3N2）virus in 100μL infectious allantoic fluid and the same volume of non-infectious allantoic fluid was given to the mock-infected mice.On the 3rd,5th,7th,10th or 14th day after inoculation,6 mice in each group were killed and the changes of pathological lesions in the lungs of the mice were observed.The other mice were used to collect bronchial alveolar lavage fluid（BALF）and serum for determining the content of NO,MDA and OH· and the activity of SOD.RESULTS： In H3N2-infected mice,alveolar and interstitial edema,hemorrhage,inflammation cell migration and infiltration were observed in the lungs,the levels of NO,MDA and OH？ in BALF dramatically increased,and the activity of SOD was dramatically decreased in BALF（P〈0.01）as compared with those in mock-infected mice（P〈0.01）.Although the changes of SOD,NO,MDA and OH· in serum had the same tendency as those in BALF,the extents of the changes were smaller.CONCLUSION：The increases in NO,MDA and OH· indicate their important roles in acute lung injury induced by H3N2 swine influenza virus in mice.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2011年第4期783-786,790,共5页
Chinese Journal of Pathophysiology
基金
河北省教育厅自然科学基金资助项目(No.2008104)
